One-step transformation with site-specific recombinase Cre/loxP system under the control of minimal 35S promoter for the development of marker-free transgenic Arabidopsis thaliana plants

1Sekan, AS
1Isaenkov, SV
1Institute of Food Biotechnology and Genomics of the NAS of Ukraine, Kyiv
Dopov. Nac. akad. nauk Ukr. 2014, 3:158-163
Section: Biochemistry
Language: Ukrainian

In a number of works, the successful use of the site-specific recombinase system Cre/loxP under the control of chemical promoters to obtain marker-free transgenic plants was described. However, providing the transformation process with the conditional or another kind of treatment to initiate the recombinase expression is the disadvantage of this method. We have developed a new approach in the site-specific recombinase system Cre/loxP to produce marker-free genetically modified plants without any treatment and shown the efficiency of Cre recombinase in the progeny of transformed plants A. thaliana.

Keywords: 35S promoter, Arabidopsis thaliana, marker-free transgenic plants, transformation

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