Sensitivity of Lewis Lung Carcinoma (LLC) and LLC/R9 cells to cytotoxic action exerted by murine natural killer cells and macrophages of C57BL/6 mice in vitro

1Fedorchuk, OG, 2Lyon, AD, 1Yakshibaeva, Yu.R, 1Gorbik, GV, 1Pyaskovskaya, ON
1R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology of the NAS of Ukraine, Kyiv
2National Cancer Institute, Kyiv
Dopov. Nac. akad. nauk Ukr. 2014, 6:167-172
Section: Medicine
Language: Ukrainian

The comparative investigation of the sensitivity of primary LLC and drug resistant LLC/R9 cells to natural killer cells and macrophages of C57BL/6 intact mice is performed in vitro. It is shown that the forming of a resistance to cisplatin by LLC cells is accompanied by an increase of their sensitivity to the cytotoxic action of natural killer cells and a decrease of the susceptibility to the cytolytic activity of macrophages. High LLC/R9 cell sensitivity to the cytotoxic action of natural killer cells possibly was caused by a diminished expression of MHC class I molecules. Decreased sensitivity of resistant cells to the cytolysis by nonsensitized macrophages was associated with a high ability to undergo autophagy.

Keywords: killer cells, Lewis lung carcinoma, macrophages of mice

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