Stimulating effect of a low-molecular fraction from cord blood on the energy metabolism in leukocytes

1Gulevsky, OK, 1Akhatova, Yu.S, 2Sysoev, AA, 2Sysoeva, IV
1Institute for Problems of Cryobiology and Cryomedicine of the NAS of Ukraine, Kharkiv
2O. O. Kovalevsky Institute of Biology of the Southern Seas, Sevastopol
Dopov. Nac. akad. nauk Ukr. 2014, 7:152-157
Section: Biochemistry
Language: Russian

The ATP, ADP, and AMP contents are determined in leukoconcentrate cells after incubation in a medium containing a low-molecular fraction (below 5 kDa) from cord blood (0.15 mg/mL) by chemiluminescent analysis. It is shown that the fraction significantly stimulates the accumulation of intracellular ATP and ADP and has no effect on the AMP content. The stimulatory effect of the low-molecular fraction from cord blood is blocked by a glycolysis inhibitor, iodoacetate Na, and a glucose transport inhibitor, cytochalasin B. It is found that the fraction from cord blood enhances the total adenylate pool of leukocytes. It is established experimentally that the presence of the low-molecular fraction from cattle cord blood stimulates the glucose accumulation in leukoconcentrate cells.

Keywords: cord blood, energy metabolism, leukocytes

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