|1Pilinskaya, MA, 1Dibskiy, SS, 1Dibskaya, Ye.B, 1Shvaiko, LI |
1The National Research Centre for Radiation Medicine of the NAMS of Ukraine, Kyiv
|Dopov. Nac. akad. nauk Ukr. 2014, 7:169-175|
The hidden chromosome instability (HCI) persistence in somatic human cells, the voluntary cytogenetic examination of 15 healthy males, who denied contact with mutagenic factors, in short- and long-term cultures of peripheral blood lymphocytes (during 48 and 100 hours, respectively) using "G2-bleomycin sensitivity assay" has been conducted. The background frequency of chromosome aberrations under the both terms of cultivation corresponded to the level of spontaneous somatic chromosome mutagenesis and did not differ significantly in terms of both cell cultures. Under the short-term incubation of lymphocytes with bleomycin, the mean-group frequency of chromosome aberrations essentially increased with considerable interindividual fluctuations that are independent of the background cytogenetic parameters. In all long-term exposed cultures, the frequency of chromosome aberrations decreased significantly in comparison with the first mitosis data, whereby not only the mean-group cytogenetic effect decreased, but the above spontaneous cytogenetic effect as well, indicating the gradual elimination of aberrant cells in successive mitosis. The intensity of the elimination of chromosome abnormalities varied considerably in different individuals and did not depend on the values of the background cytogenetic effect in intact cultures and on the individual levels of chromosome aberrations induced by bleomycin in short-term exposed cultures. The data confirmed the importance of genetically determined interindividual peculiarities for the induction and the persistence of HCI in somatic human cells.
|Keywords: chromosome instability, lymphocytes, peripheral blood, persistence|
1. Spitz M. Cancer Detect. Prev., 2005, 19, No. 1: 35.
2. Atkinson M. Individual sensitivity Retrieved from http://www.nea.fr/html/rp/helsinki08/presentations.
3. Pilinska M. A., Dybskyi S. S., Dybska O. B. ,Pedan L. R. Dopov. Nac. akad. nauk Ukr., 2008, No. 8: 184–188 (in Ukraianian).
4. Pilinska M. A., Dybskyi S. S., Dybska O. B., Pedan L. R. Tsitologiia i genetika, 2010, 44, No. 2: 58–64 (in Ukrainian).
5. Pilinska M. A., Dybskyi S. S., Dybska O. B., Shvaiko L. I. Tsitologiia i genetika, 2012, No. 6: 36-43 (in Russian).
6. Pilinska M. A., Dybskyi S. S., Dybska O. B., Shvaiko L. I., Sushko V. O. Dopov. Nac. akad. nauk Ukr., 2012, No. 10: 156–161 (in Ukrainian).
7. Pat. 64932 UA, IPC G01N33/49. Method fore diagnostics of hidden chromosomal instability in human blood lymphocytes. Pilinska M. A., Dybskyi S. S., Dybska O. B. Publ. 25.11.2011 (in Ukrainian).
8. Pat. 15062 UA, IPC C12Q1/68, G01N33/48. Method for detecting instability of the chromosomes in the blood lymphocytes of the descendants of irradiated human beings. Pilinska M. A., Dybskyi S. S. Publ. 15.06.2006 (in Ukrainian).
9. Bochkov N. P., Chebotarev A. N., Katosova L. D., Platonova V. I. Vestn. RAMN, 2001, No. 2: 21–29 (in Russian).
10. Pilinska M. A., Dybskyi S. S. Tsitologiia i genetika, 2004, 40, No. 4: 61–66 (in Russian).
11. Pilinska M. A., Dybskyi S. S., Dybska O. B. et al. Tsitologiia i genetika, 2004, 40, No. 5: 23-28 (in Russian).
12. Chebotarev A. N. Vestn. RAMN, 2001, No. 10: 64–69 (in Russian).
13. Pilinska M. A., Dybskyi S. S., Dybska O. B., Pedan L. R. Tsitologiia i genetika, 2005, 39, No. 4: 32-40 (in Russian).
14. Shemetun O. V., Talan O. O., Pilinska M. A. The problem of sustainability of human chromosomes under the action of ionizing radiation due to the bystander effect. Proceedings of the XIII Congress of the World Federation of Medical Associations, 2010, Lviv; Kyiv; Chikago, 2010: 699 (in Ukrainian).