Comparative taxonomic analysis of Pseudomonas batumici and evolutionally related species

1Klochko, VV
1D.K. Zabolotny Institute of Microbiology and Virology of the NAS of Ukraine, Kyiv
Dopov. Nac. akad. nauk Ukr. 2015, 2:152-157
Section: Biochemistry
Language: Russian

Results of 16S rRNA gene sequences analysis have been shown that strain Pseudomonas batumici UCM V-321T forms a separate branch within the genus Pseudomonas and has 98% of 16S rRNA gene sequence similarity with evolutionally most related species P. gingeri and P. baetica. The differences between the mentioned species determined by the polyphasic taxonomic analysis include the presence of some enzymes, pigments production, spectra of assimilated carbon sources, fatty acid profiles, and antagonistic activity. Synthesis of antistaphylococcal antibiotic batumin was found only in P. batumici strains and is, probably, the unique peculiarity of this species.

Keywords: antibiotic, antistafilokok, batumin, Pseudomonas, Pseudomonas batumici, synthesis
  1. Silby M., Winstanley C., Godfrey S. et al. Pseudomonas genomes: diverse and adaptable, FEMS Microbiol. Rev., 2011, 35, no.4: 652–680.
  2. Palleroni N. The Pseudomonas Story, Environ. Microbiol., 2010, 12, no.6: 1377–1383.
  3. Kiprianova E. A., Klochko V. V., Zelena L. B. et al. Pseudomonas batumici sp. nov., the antibiotic-producing bacteria isolated from soil of the Caucasus Black Sea coast, Mikrobiol. zhurn., 2011, 73, no.5: 3–8.
  4. Wong W., Fletcher J., Unsworth B., Preece T. A note on ginger blotch, a new bacterial disease of the cultivated mushroom, Agaricus bisporus, J. Appl. Bacteriol., 1982, no.52: 43–48.
  5. Lopez J., Dieguez A., Doce A. et al. Pseudomonas baetica sp. nov., a fish pathogen isolated from wedge sole, Dicologlossa cuneata (Moreau), Int. J. Syst. Evol. Microbiol., 2012, no.62: 874–882.
  6. Kiprianova E. A., Churkina L. N., Klochko V. V., Prihod'ko V. A. Original'nyj otechestvennyj antibiotik batumin: struktura i aktivnost', biotehnologija, perspektivy ispol'zovanija v medicine, Materialy VI Nats. z'izdu farmatsevtiv Ukrainy "Dosiahnennia ta perspektyvy rozvytku farmatsevtychnoi haluzi Ukrainy", Kharkiv, 2005 [in Russian].
  7. Patent of Ukraine no.23609 A, MPK C12N 1/20. Smirnov, V.V., Kiprianova O.A., Churkina L.M., Harahulia O.D., Mukvych M.S.: A Strain Pseudomonas Batumici 17 Producer of anti staphylococcus antibiotic of batumin, no.31, 02/06/1998.
  8. Safronova L. A., Zelena L. B., Klochko V. V., Reva O. N. Does the applicability of Bacillus strains in probiotics rely upon their taxonomy?, Can. J. Microbiol., 2012, no.58: 212–219.
  9. Tamura K., Peterson D., Peterson N. et al. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony, Meth. Mol. Biol. and Evol., 2011, no.28: 2731–2739.
  10. Cutri S., MacCauley B., Roberts W. Characteristics of pathogenic non-fluorescent (smooth) and nonpathogenic fluorescent (rough) forms of Pseudomonas tolaasii and P. gingeri, J. Appl. Bacteriol., 1984, no.57: 291–298.