Stimulating effect of a low-molecular fraction (below 5 kDa) from cord blood on glycogenolysis in neutrophils of human donor blood leukoconcentrate

1Gulevskyy, OK
1Akhatova, Yu.S
1Institute for Problems of Cryobiology and Cryomedicine of the NAS of Ukraine, Kharkiv
Dopov. Nac. akad. nauk Ukr. 2015, 7:123-128
Section: Biology
Language: Russian

A semiquantitative evaluation of the glycogen content in neutrophils of freshly isolated and cryopreserved leukoconcentrates subjected to the incubation in a medium containing a low-molecular fraction (below 5 kD) from cord blood (0.15 mg/ml) is performed. It is found that the fraction contributed to glycogenolysis in cryopreserved neutrophils, which is manifested as a significant reduction in the mean cytochemical coefficient. It is shown that the effect of the low-molecular fraction from cord blood on glycogenolysis is blocked by a glycolysis inhibitor, sodium iodoacetate. The obtained data reveal a mechanism of the stimulating effect of the low-molecular fraction from cord blood on the energy metabolism of leukoconcentrate cells.

Keywords: cord blood, cryopreservation, glycogen, neutrophils
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