Stimulating effect of a low-molecular fraction (below 5 kDa) from cord blood on glycogenolysis in neutrophils of human donor blood leukoconcentrate

1Gulevskyy, OK
1Akhatova, Yu.S
1Institute for Problems of Cryobiology and Cryomedicine of the NAS of Ukraine, Kharkiv
Dopov. Nac. akad. nauk Ukr. 2015, 7:123-128
https://doi.org/10.15407/dopovidi2015.07.123
Section: Biology
Language: Russian
Abstract: 

A semiquantitative evaluation of the glycogen content in neutrophils of freshly isolated and cryopreserved leukoconcentrates subjected to the incubation in a medium containing a low-molecular fraction (below 5 kD) from cord blood (0.15 mg/ml) is performed. It is found that the fraction contributed to glycogenolysis in cryopreserved neutrophils, which is manifested as a significant reduction in the mean cytochemical coefficient. It is shown that the effect of the low-molecular fraction from cord blood on glycogenolysis is blocked by a glycolysis inhibitor, sodium iodoacetate. The obtained data reveal a mechanism of the stimulating effect of the low-molecular fraction from cord blood on the energy metabolism of leukoconcentrate cells.

Keywords: cord blood, cryopreservation, glycogen, neutrophils
References: 
  1. Gulevsky O.K., Akhatova Yu. S., Sysoev A.A., Sysoeva I.V. Dopov. Nac. akad. nauk Ukr., 2014, No 7: 152–157 (in Ukrainian).
  2. Grishina V.V., Timokhina Ye.V., Andreeva L.Yu. Voprosy Ginekologii, Akusherstva i Perinatologii, 2004, 3, No 6: 50–54 (in Russian).
  3. Gulevsky O.K., Akhatova Yu.S., Moiseeva N.N., Gorina O. L., Chizhevsky V.V., Stepanyuk L.V. Problems of Cryobiology, 2012, 22, No 1: 71–79 (in Russian).
  4. Brock T.D. Membrane filtration: a user's guide and reference manual, Madison, WI: Science Tech., 1983. https://doi.org/10.1007/978-3-662-08650-6
  5. Medical immunobiological preparations: Methodological recommendations on the standard operating procedures for blood products, Resolution on Quality 42–3002–011–2005, Kyiv: Ministry of Health of Ukraine, 2005. Directive No 376 of Ministry of Health of Ukraine dd 26.07.2005 (in Ukrainian).
  6. Clinical laboratory analytics. Vol. 2. Some analytical techniques in the clinical laboratory, Ed. by V.V.Menshikov, Moscow: RAMLD, 1999 (in Russian).
  7. Pochtar M.E., Lugovskaya S.A., Morozova V.T. Cytochemical diagnostics in laboratory hematology: Methodological manual, Atlas, St.-Petersburg: Triada, 2003 (in Russian).
  8. Borregaard N., Herlin T. J. Clin. Invest., 1982, 70, No 9: 550–557. https://doi.org/10.1172/JCI110647
  9. Robinson J.M., Karnovsky M. L., Karnovsky M. J. J. Cell. Biol., 1982, 95, No 3: 933–942. https://doi.org/10.1083/jcb.95.3.933
  10. Afonso A., Macedo P.M., Ellis A.E., Silva M.T. Dis. Aquat. Organ., 2000, 42, No 2: 101–110. https://doi.org/10.3354/dao042101
  11. Chou J.Y., Jun H. S., Mansfield B.C. Curr. Opin. Hematol., 2010, 17, No 1: 36–42. https://doi.org/10.1097/MOH.0b013e328331df85
  12. Jun H. S., Weinstein D.A., Lee Y.M., Mansfield B.C., Chou J.Y. Blood, 2014, 123, No 18: 2843–2853. https://doi.org/10.1182/blood-2013-05-502435
  13. Nazarenko G. I., Kiskun A.A. Clinical evaluation of laboratory results, Moscow: Meditsina, 2007 (in Russian).
  14. Utemov S.V. Low temperature (−80 ºC) preservation of leukocyte concentrates: Synopsis of thesis for the degree of Candidate of Medical Sciences, St.-Petersburg, 2005 (in Russian).