|1Kartel, NT, 1Ivanov, LV, 2Lyapunov, AN, 3Nardid, OA, 3Cherkashina, Ya.O, 4Gurova, OA, 5Ocotrub, AV |
1O. O. Chuiko Institute of Surface Chemistry of the NAS of Ukraine, Kyiv
2State Scientific Institution "Institute for Single Crystals" of the NAS of Ukraine, Kharkiv
3Institute for Problems of Cryobiology and Cryomedicine of the NAS of Ukraine, Kharkiv
4Nikolaev Institute of Inorganic Chemistry of the SB of RAS, Novosibirsk, Russia
5Nikolaev Institute of Inorganic Chemistry of the SB of RAS, Novosibirsk, Russia; Novosibirsk State University, Russia
|Dopov. Nac. akad. nauk Ukr. 2017, 12:73-82|
By the method of spin probes, we have studied the effect of initial and oxidized carbon nanoshorns on the microviscosity of rat erythrocyte membranes and the viscosity of the water-containing plasma protein matrix. Introduction of nanohorns in a concentration of 100 μg/ml into erythrocyte suspension results in an increase in the membrane microviscosity within four hours (about 60 % effect), increasing the polarity of a microenvironment for lipophilic probes in the outer layer of membrane phospholipids, and a disorder of erythrocyte's membranes. Introduction of nanohorns in plasma leads to a slight reduction in the viscosity of water and the protein matrix, apparently, due to its partial destruction, especially macromolecules of serum albumin. Cytotoxicity of pristine and oxidized nanohorns evaluated by us is higher as compared to the nanoparticles of oxidized graphene, but is significantly lower than the carbon nanotubes, which are capable of dramatically increasing the microviscosity of membranes of erythrocytes and disrupting their integrity.
|Keywords: anisotropy of SPR spectra, carbon nanochorns, cytotoxicity, method of spin probes, microviscosity of erythrocyte membranes, polarity of the microenvironment of a probe in the membrane, serum albumin|
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