The lack of modifying effect of astaxanthin on cytogenetic effects in irradiated in vitro human peripheral blood lymphocytes on stage G2 of the cell cycle

1Kurinnyi, DA, 2Rushkovsky, SR, 1Pilinska, MA
1National Research Center for Radiation Medicine of the NAMS of Ukraine, Kyiv
2Institute of Biology and Medicine, Taras Shevchenko National University of Kyiv
Dopov. Nac. akad. nauk Ukr. 2017, 4:91-95
https://doi.org/10.15407/dopovidi2017.04.091
Section: Genetics
Language: Ukrainian
Abstract: 

Radioprotective potential of astaxanthin after gamma-irradiation of human peripheral blood lymphocytes' cultures in vitro in dose 1.0 Gy on the post synthetic (G2) stage of the first mitotic cycle has been studied. The lack of astaxanthin modifying effect on stage G2 has been demonstrated. No significant changes in the total frequency of chromosomal disorders and in the spectrum of chromosomal aberrations are detected. The comparison of astaxanthin effects between stage G2 and G0 of the cell cycle is conducted.

Keywords: astaxanthin, chromosome aberrations, culture of human peripheral blood lymphocytes, radioprotective effect
References: 
  1. Stewart, J. S., Lignell, A., Pettersson, A., Elfving, E. & Soni, M. G. (2008). Safety assessment of astaxanthin-rich microalgae biomass: Acute and subchronic toxicity studies in rats. Food Chem. Toxicol., 46, Iss. 9, pp. 3030-3036. https://doi.org/10.1016/j.fct.2008.05.038
  2. Pilinska, M. A., Kurinnyi, D. A., Rushkovsky, S. R. & Dybska, O. B. (2016). The impact of astaxanthin on radiation-induced chromosome aberrations in human peripheral blood lymphocytes in vitro. Visn. Ukr. tov-va genetykiv i selektsioneriv, 14, No. 1, pp. 52-57 (in Ukrainian).
  3. Sanford, K. K., Parshad, R., Price, F. M., Jones, G. M., Tarone, R. E., Eierman, L., Hale, P. & Waldmann, T. A. (1990). Enhanced Chromatid Damage in Blood Lymphocytes After G 2 Phase X Irradiation, a Marker of the Ataxia-Telangiectasia Gene. J. Natl. Cancer Inst., 82, pp. 1050-1055. https://doi.org/10.1093/jnci/82.12.1050
  4. Dyomina, E. A. & Ryabchenko, N. M. (2007). Increased individual chromosomal radiosensitivity of human lymphocytes as a parameter of cancer risk. Exp. Oncol., 29, pp. 217-220.
  5. Ryabchenko, N. M., Glavin, O. A., Shtefura, V. V. & Anikushko, M. F. (2013). Chromosomal radiosensitivity in Ukrainian breast cancer patients and healthy individuals. Exp. Oncol., 34, No. 2, pp. 121-124.
  6. Baert, A., Depuydt, J., Van Maerken T., Poppe, B., Malfait, F., Storm, K., Van den Ende, J., Van Damme, T., De Nobele, S., Perletti, G., De Leeneer, K., Claes, K. B. M. & Vral, A. (2016). Increased chromosomal radiosensitivity in asymptomatic carriers of a heterozygous BRCA1 mutation. Breast Cancer Res., 18:52, 12 p. doi: https://doi.org/10.1186/s13058-016-0709-1.
  7. Pedan, L. R. & Pilinska, M. A. (2004). Assessment of the stability of chromosomes of peripheral blood lymphocytes of victims of the Chernobyl accident factors using testing mutagenic burden in vitro. Reports of the National Academy of Sciences of Ukraine, No. 5, pp. 175-179 (in Ukrainian).
  8. Zakharov, A.F., Benyush, V.A., Kuleshov, N.P. & Baranowska, L.I. (1982). Human Chromosomes: Atlas. Mossow: Meditsina (in Russian).