Genomics and virion DNA structure of temperate erwiniophages 49 and 59

1Zlatohurska, MA, 2Tovkach, FI
1I. I. Mechnikov Odessa National University
2D.K. Zabolotny Institute of Microbiology and Virology of the NAS of Ukraine, Kyiv
Dopov. Nac. akad. nauk Ukr. 2017, 6:82-87
https://doi.org/10.15407/dopovidi2017.06.082
Section: Biology
Language: Russian
Abstract: 

A comparative study of virion DNAs of phages 49 and 59 by the standard restriction and bioinformatic analyses is conducted. It has been shown that the genomes of both bacteriophages are cyclically permuted, but phage 59 is characterized by continuous permutation of the genome, while phage 49 has discrete permutation. Virion DNA of phage 49 has an abnormal cleavage site for SmaI restriction enzyme that, unlike the rest SmaIsites, is not completely hydrolyzed.

Keywords: bacteriophages 49 and 59, bioinformatic analysis, circular and discrete permutation, headful packaging mechanism, restriction analysis
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