Induction of NO synthesis in roots of wheat plantlets and development of their heat resistance by exogenous L-arginine and nitrate

1Karpets, Yu.V, 1Kolupaev, Yu.E, 2Dmitriev, AP
1V. V. Dokuchaev National Agrarian University of Kharkiv
2Institute of Cell Biology and Genetic Engineering of the NAS of Ukraine, Kyiv
Dopov. Nac. akad. nauk Ukr. 2017, 7:77-84
Section: Biology
Language: Russian

The treatment of roots of intact plantlets of wheat (Triticum aestivum L.) with L-arginine and sodium nitrate caused an increase of the content of nitric oxide (NO) in them and raised the resistance to the damaging heating. The arginine-dependent increase of the NO content was suppressed by the pretreatment of roots with NO-synthase inhibitor L-NAME (NG-nitro-L-arginine methyl ester), and the nitrate-dependent one — with nitrate reductase inhibitor, sodium tungstate. These inhibitors eliminated also the positive influence of exogenous L-arginine and nitrate on the heat resistance of plantlets that confirms the crosstalk of such influence with the process of nitric oxide synthesis. At the combined treatment of plantlets with L-arginine and nitrate, their influence on the content of nitric oxide in roots and the development of the heat resistance of plantlets was leveled. The oppression of the nitrate-dependent formation of nitric oxide in plantlets roots, caused by L-arginine, was partially removed by NO-synthase inhibitor L-NAME. Thus, the data on the antagonism of the arginine- and nitrate-dependent pathways of nitric oxide synthesis in plant cells are obtained for the first time.

Keywords: heat resistance, L-arginine, nitrate, nitrate reductase, nitric oxide, NO-synthase, Triticum aestivum
  1. Dmitriev, A. P. (2004). Sygnal role of nitric oxide in plants. Tsitol. Genet., 38, No. 4, pp. 67-75 (in Russian).
  2. Wilson, I. D., Neill, S. J. & Hancock, J. T. (2008). Nitric oxide synthesis and signalling in plants. Plant Cell Environ., 31, No. 5, pp. 622-631.
  3. Khan, M. N., Mobin, M. & Abbas, Z. K. (2015). Nitric oxide and high temperature stress: a physiological perspective. In Khan, M. N. et al. (Eds.). Nitric oxide action in abiotic stress responses in plants (rr. 77-94). Heidelberg; New York; Dordrecht; London: Springer. doi:
  4. Karpets, Yu. V., Kolupaev, Yu. E. & Vauner, A. A. (2015). Functional interaction between nitric oxide and hydrogen peroxide during formation of wheat seedling induced heat resistance. Russ. J. Plant Physiol., 62, No. 1, pp. 65-70.
  5. Siddiqui, M. H., Al-Whaibi, M. H. & Basalah, M. O. (2011). Role of nitric oxide in tolerance of plants to abiotic stress. Protoplasma, 248, No. 3, pp. 447-455.
  6. Barand, A., Nasibi, F. & ManouchehriKalantari, Kh. (2015). The effect of arginine pretreatment in the increase of coldtolerance in Pistacia vera L. in vitro. Russ. Agricult. Sci., 41, No. 5, pp. 340-346.
  7. Mur, L. A. J., Mandon, J., Persijn, S., Cristescu, S. M., Moshkov, I. E., Novikova, G. V., Hall, M. A., Harren, F. J. M., Hebelstrup, K. H. & Gupta, K. J. (2013). Nitric oxide in plants: an assessment of the current state of knowledge. AoB Plants, 5, pls052.
  8. Glyan'ko, A. K. & Mitanova, N. B. (2011). Synthesis nitric oxide (NO) in roots etiolated seedlings of pea. Visn. Kharkiv Nat. Agr. Un-ty. Ser. Biol., Iss. 3, pp. 6-14 (in Russian).
  9. Roszer, T. (2014). Biosynthesis of nitric oxide in plants. In Khan, M. N. et al. (Eds.). Nitric Oxide in Plants: Metabolism and Role in Stress Physiology (pp. 17-32). Cham: Springer.
  10. Corpas, F. J. & Barroso, J. B. (2016). Nitric oxide synthase-like activity in higher plants. Nitric Oxide. doi:
  11. Crawford, N. M. (2005). Mechanisms for nitric oxide synthesis in plants. J. Exp. Bot., 57, No. 3, pp. 471-478. doi:
  12. Shi, F.-M. & Li, Y.-Z. (2008). Verticillium dahliae toxins-induced nitric oxide production in Arabidopsis is major dependent on nitrate reductase. BMB Rep., 41, No. 1, pp. 79-85.
  13. Karpets, Yu. V., Kolupaev, Yu. E., Yastreb, T. O. & Oboznyi, A. I. (2016). Induction of heat resistance in wheat seedlings by exogenous calcium, hydrogen peroxide, and nitric oxide donor: functional interaction of signal mediators. Russ. J. Plant Physiol., 63, No. 4, pp. 490-498.
  14. Rosales, E. P., Iannone, M. F., Groppa, M. D. & Benavides, M. P. (2011). Nitric oxide inhibits nitrate reductase activity in wheat leaves. Plant Physiol. Biochem., 49, No. 2, pp. 124-130.
  15. Vital, S. A., Fowler, R. W., Virgen, A., Gossett, D. R., Banks, S. W. & Rodriguez, J. (2008). Opposing roles for superoxide and nitric oxide in the NaCl stress-induced upregulation of antioxidant enzyme activity in cotton callus tissue. Environ. Exp. Bot., 62, No. 1, pp. 60-68.