Induction of NO synthesis in roots of wheat plantlets and development of their heat resistance by exogenous L-arginine and nitrate

1Karpets, Yu.V, 1Kolupaev, Yu.E, 2Dmitriev, AP
1V. V. Dokuchaev National Agrarian University of Kharkiv
2Institute of Cell Biology and Genetic Engineering of the NAS of Ukraine, Kyiv
Dopov. Nac. akad. nauk Ukr. 2017, 7:77-84
https://doi.org/10.15407/dopovidi2017.07.077
Section: Biology
Language: Russian
Abstract: 

The treatment of roots of intact plantlets of wheat (Triticum aestivum L.) with L-arginine and sodium nitrate caused an increase of the content of nitric oxide (NO) in them and raised the resistance to the damaging heating. The arginine-dependent increase of the NO content was suppressed by the pretreatment of roots with NO-synthase inhibitor L-NAME (NG-nitro-L-arginine methyl ester), and the nitrate-dependent one — with nitrate reductase inhibitor, sodium tungstate. These inhibitors eliminated also the positive influence of exogenous L-arginine and nitrate on the heat resistance of plantlets that confirms the crosstalk of such influence with the process of nitric oxide synthesis. At the combined treatment of plantlets with L-arginine and nitrate, their influence on the content of nitric oxide in roots and the development of the heat resistance of plantlets was leveled. The oppression of the nitrate-dependent formation of nitric oxide in plantlets roots, caused by L-arginine, was partially removed by NO-synthase inhibitor L-NAME. Thus, the data on the antagonism of the arginine- and nitrate-dependent pathways of nitric oxide synthesis in plant cells are obtained for the first time.

Keywords: heat resistance, L-arginine, nitrate, nitrate reductase, nitric oxide, NO-synthase, Triticum aestivum
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