Comparative physical chemical stability of composition systems of hydroxyapatite/polyethyleneglycol 400 and 6000 in biological media

1Boshytska, NV, 1Protsenko, LS, 1Budilina, ON, 1Kaplunenko, NV, 1Uvarova, IV
1I. M. Frantsevich Institute for Problems of Materials Sciences of the NAS of Ukraine, Kyiv
Dopov. Nac. akad. nauk Ukr. 2017, 8:43-50
https://doi.org/10.15407/dopovidi2017.08.043
Section: Materials Science
Language: Ukrainian
Abstract: 

Interaction of composite systems on the bases of hydroxyapatite and polyethylene glycol (HAP + PEG) with molecular weights of 400 and 6000 with physiological Ringer and Ringer-Locke solution of NaCl has been investigated. By the methods of chemical analysis, it is established that the HAP + PEG 400 composite system interacts with biological media liberating calcium from a material step-by-step. The powder HAP + PEG 6000 system remains chemically stable during 100 h. By IR-spectroscopy, it is demonstrated that the spectrogram of surfaces of HAP + PEG 400 and HAP + PEG 6000 samples after the interaction with physiological solutions during 100 h contains valence vibrations in a range corresponding to the PEG presence. It can be supposed that the chemical activity of HAP + PEG 400 material relative to calcium and the prolonged presence of polyethylene glycol in the biological media help a reduction in both mineral metabolism and nervous impulses at bone defects. It is shown that the composite HAP + PEG 400 system is promising for the future development of materials for orthopedic applications.

Keywords: chemical stability, hydroxyapatite, physiological solution, polyethylene glycol, surface
References: 
  1. Delgado, C., Francis, G. & Fisher, D. (1992). The Uses and Properties of PEG-Linked Proteins. Crit. Rev. Ther. Drug Carrier Syst., 9 (3/4), pp. 249-304.
  2. Bruce, A. (2001). Clinical considerations in pegylated protein therapy. From Research to Practice, 3 (1), pp. 3-9.
  3. Batiza, R. & White, J. D. L. (1999). Submarine Lavas and Hyaloclastite. Encyclopedia of Volcanoes; 1417 p. Ed. H. Sigurdsson. New York: Academic Press.
  4. Garratty, G. (2008). Modulating the Red Cell Membrane to Produce Universal/Stealth Donor Red Cells Suitable for Transfusion. Vox Sanguinis, 94, No. 2, pp. 87-95.
  5. Pertsiv, I.M, Datsenko, B. M, Gunko, V. Y. (1991). Development of drugs multidirectional action on purulent inflammation: study of manufacturing, clinical experience and application. Pha. Zh., No. 3, pp. 5; 56-61; 65-68 (in Ukrainian).
  6. Jaiswal, J., Gupta, S. K. & Kreuter, J. (2004). Preparation of Biodegradable Cyclosporine Nanoparticles by High-Pressure Emulsion-Solvent Evaporation Process. J. Control. Release, No. 96, pp. 169-178. https://doi.org/10.1016/j.jconrel.2004.01.017
  7. Bittner, G. D. et al. (2012). Rapid, Effective, and Long-Lasting Behavioral Recovery Produced by Micro sutures, Methylene Blue, and Polyethylene Glycol after Completely Cutting Rat Sciatic Nerves. J. Neuroscience Research, 90 (5), pp. 967-980. https://doi.org/10.1002/jnr.23023
  8. Bittner, G. D. et al. (2005). Melatonin Enhances the in vitro and in vivo Repair of Severed Rat Sciatic Axons. Neuroscience Letters, 376 (2), pp. 98-101. https://doi.org/10.1016/j.neulet.2004.11.033
  9. Britt, J. M., Kane, J. R., Spaeth, C. S. et al. (2010). Polyethylene Glycol Rapidly Restores Axonal Integrity and Improves the Rate of Motor Behavior Recovery after Sciatic Nerve Crush Injury. J. Neurophysiology, 104 (2), pp. 695-703. https://doi.org/10.1152/jn.01051.2009
  10. Sexton, K. W., Pollins, A. C., Cardwell, N. L. et al. (2012). Polyethylene Glycol Rapidly Restores Axonal Integrity and Improves the Rate of Motor Behavior Recovery after Sciatic Nerve Crush Injury. J. Surgical Research, 177 (2), pp. 392-400. https://doi.org/10.1016/j.jss.2012.03.049
  11. Pentin, Yu. A. & Vilkov, L. (1987). Physical methods of research in chemistry. Moscow: Higher School (in Russian).
  12. Liopo, V. A., Himpel, N. N. & Vasyl'yev, Ye. K. (1995). X-ray phase analysis using a database. X-ray application in science and technology.Irkutsk. State. Univ., pp. 125-131 (in Russian).
  13. Krylov, A. A, Kats, A. M and others. (1981). Manual for clinical diagnostic laboratories. Leningrad: Medicine (in Russian).