Influence of UV-B on expression profiles of genes involved in the development of autophagy by means of microtubules

Olenieva, VD
Lytvyn, DI
1Yemets, AI
1Blume, Ya.B
1Institute of Food Biotechnology and Genomics of the NAS of Ukraine, Kyiv
Dopov. Nac. akad. nauk Ukr. 2018, 1:100-109
Section: Biology
Language: Ukrainian

Transcriptome analysis of key genes involved in realization of autophagy induced by UV-B irradiation in Arabidopsis thaliana cells is conducted. Changes in expression of α-tubulin and atg8 genes had clearly defined doseand time-dependent nature. Overexpression of tub4 and atg8a shown at time points of 3 and 24 h after irradiation indirectly testifies to the interaction between the structural units of autophagosomes and microtubules. Expression profiles of elp3/deacetylases, as well as hexokinases, denote the crucial role of α-tubulin acetylation for autophagic response - a stage preceding the development of programmed cell death.

Keywords: atg8, autophagy, programmed cell death, transcriptome analysis, UV-B, α-tubulin
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