The obtaining of tomato and potato plants with human lactoferrin gene hLF

Buziashvili, AY, 1Yemets, AI
1Institute of Food Biotechnology and Genomics of the NAS of Ukraine, Kyiv
Dopov. Nac. akad. nauk Ukr. 2018, 10:88-94
Section: Biology
Language: Ukrainian

The agrobacterium-mediated transformation of different genotypes of potato (Solanum tuberosum) and tomato (Lycopersicon esculentum) with human lactoferrin gene is conducted. Genetically modified lines of potato (Ukrainian varieties Levada and Svitanok) and tomato (Money Maker and Ukrainian variety Lahidnyi) are obtained and analyzed. The transfer and incorporation of hLF gene into respective plant genomes was confirmed by the polymerase chain reaction with specific primers to lactoferrin gene. Preliminary biotests for the phytopato gene resistance are conducted, and a higher resistance of transgenic lines to Phytophthoraas is found.

Keywords: agrobacteriummediated transformation, human lactoferrin gene, polymerase chain reaction, potato, tomatoes, transgenic lines
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