Phytohormonal regulation of the development of Dryopteris filix-mas (L.) Schott gametophyte in vitro culture

1Romanenko, KO, 1Babenko, LM, 2Vasheka, OV, 2Romanenko, PO, 1Kosakivska, IV
1M. G. Kholodny Institute of Botany of the NAS of Ukraine, Kyiv
2Institute of Biology and Medicine, Taras Shevchenko National University of Kyiv
Dopov. Nac. akad. nauk Ukr. 2018, 11:96-105
Section: Ecology
Language: Ukrainian

Effects of exogenous cytokinin phytohormones such as kinetin, zeatin, 6-benzylaminopurine, and N6-2-isopentenyladenine on the gametophyte morphology and growth features of Dryopteris filix-mas (L.) Schott in vitro culture have been studied. It is established that, at the concentration of 10−5 M, all studied cytokinins inhibit the gametophyte growth, cause deformations and changes in the thallus size, and suppress the development of reproductive structures and sporophyte growth. Reduction of the hormone concentration to 10−8 M stimulates the gametophyte development, induces cell divisions, particularly in the apical zone, due to which some of thalli are deformed, promotes the production of rhizoids, affects the formation of antheridia and archegonia, and slows the sporophyte development.

Keywords: 6-benzylaminopurine, cytokinins, Dryopteris filix-mas, gametophyte, kinetin, N6-2-isopentenyladenine, prothallium, spores, thallus, zeatin
  1. Kosakivska, I. V., Babenko, L. M., Shcherbatiuk, M. M., Vedenicheva, N. P., Voytenko, L. V. & Vasyuk, V. A. (2016). Phytohormones during growth and development of Polypodiophyta. Adv. Biol. Earth Sci., 1, pp. 26-44.
  2. Davies, P. J. (2010). Plant hormones: biosynthesis, signal transduction, action. Berlin: Springer. doi:
  3. Takeno, K. & Furuya, M. (1977). Inhibitory effect of gibberellins on archegonial differentiation in Lygodium japonicum. Physiol. Plant., 39, pp. 135-138. doi:
  4. Swami, P. & Raghavan, V. (1980). Control of morphogenesis in the gametophyte of a fern by light and growth hormones. Can. J. Bot., 58, pp. 1464-1473. doi:
  5. Kaźmierczak, A. (2003). Induction of cell division and cell expansion at the beginning of gibberellin A3-induced precocious antheridia formation in Anemia phyllitidis gametophytes. Plant Sci., 165, pp. 933-939. doi:
  6. Camloh, M., Ravnikar, M. & Zel, J. (1996). Jasmonic acid promotes division of fern protoplasts, elongation of rhizoids and early development of gametophytes. Physiol. Plant., 97, pp. 659-664. doi:
  7. Kwa, S. H., We,e Y. C., Lim, T. M. & Kumar, P. P. (1995). IAA-induced apogamy in Platycerium coronarium (Koenig) Desv. gametophytes cultured in vitro. Plant Cell Rep., 14, pp. 598-602. doi:
  8. Menéndez, V., Revilla, M. A., Fal, M. A. & Fernández, H. (2009). The effect of cytokinins on growth and sexual organ development in the gametophyte of Blechnum spicant L. Plant Cell Tiss. Organ Cult., 96, pp. 245-250. doi:
  9. Vedenicheva, N. P. & Kosakivska, I. V. (2017). Cytokinins as regulators of plant ontogenesis under different growth conditions. Kiev: Nash Format (in Ukrainian).
  10. Bonomo, M. C., Martínez, O. G., Tanco, M. E., Cardozo, R. & Avilés, Z. (2013). Spores germination and gametophytes of Alsophila odonelliana (Cyatheaceae) in different sterile media. Phyton (Buenos Aires), 82, pp. 119-126.
  11. Greer, G. K., Dietrich, M. A., DeVol, J. A. & Rebert, A. (2012). The effects of exogenous cytokinin on the morphology and gender expression of Osmunda regalis gametophytes. Amer. Fern J., 102, pp. 32-46. doi:
  12. Spiro, M. D., Torabi, B. & Cornell, C. N. (2004). Cytokinins induce photomorphogenic development in darkgrown gametophytes of Ceratopteris richardii. Plant Cell Physiol., 45, pp. 1252-1260. doi:
  13. Babenko, L. M., Romanenko, K. O., Shcherbatiuk, M. M., Vasheka, O. V., Romanenko, P. O., Negretsky, V. A. & Kosakivska, I. V. (2018). Effects of exogenous phytohormones on spore germination and morphogenesis of Polystichum aculeatum (L.) Roth gametophyte in vitro culture. Cytol. Genet., 52, pp. 117-126. doi:
  14. Nayar, B. K. & Kaur, S. (1971). Gametophytes of Homosporous Ferns. Bot. Rev., 37, pp. 295-396. doi:
  15. Gaba, V. (2005). Plant growth regulators in plant tissue culture and development. In Plant tissue culture, development, and biotechnology (pp. 87-99.), CRC Press.