Adaptor protein Ruk/CIN85 modulates manifestation of cancer stem cells (CSCs) features in mouse breast adenocarcinoma 4T1 cells

Horak, IR
Pasichnyk, GV
Gerashchenko, DS
Knopfova, L
Borsig, L
Drobot, LB
Dopov. Nac. akad. nauk Ukr. 2018, 12:101-109
Section: Biochemistry
Language: Ukrainian

Using mouse breast adenocarcinoma 4T1 cells with stable overexpression or knockdown of adaptor protein Ruk/CIN85, its contribution to the manifestation of cancer stem cells features was estimated. It was demonstrated that the expression levels of reprogramming factors Klf4, Pou5f1/Oct4 and Nanog, as well as CSCs surface markers Cd44 and Cd24, depended on Ruk/CIN85 expression level: increased in the case of up-regulation and decreased in the case of down-regulation. It was found that the transcriptome patterns in 4T1 subclones with different expression levels of Ruk/CIN85 correlated with their ability to form mammospheres.

Keywords: adaptor protein Ruk/CIN85, breast adenocarcinoma, cancer stem cells, reprogramming, spheroids
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