Adaptor protein Ruk/CIN85 modulates manifestation of cancer stem cells (CSCs) features in mouse breast adenocarcinoma 4T1 cells

Horak, IR, Pasichnyk, GV, Gerashchenko, DS, Knopfova, L, Borsig, L, Drobot, LB
Dopov. Nac. akad. nauk Ukr. 2018, 12:101-109
https://doi.org/10.15407/dopovidi2018.12.101
Section: Biochemistry
Language: Ukrainian
Abstract: 

Using mouse breast adenocarcinoma 4T1 cells with stable overexpression or knockdown of adaptor protein Ruk/CIN85, its contribution to the manifestation of cancer stem cells features was estimated. It was demonstrated that the expression levels of reprogramming factors Klf4, Pou5f1/Oct4 and Nanog, as well as CSCs surface markers Cd44 and Cd24, depended on Ruk/CIN85 expression level: increased in the case of up-regulation and decreased in the case of down-regulation. It was found that the transcriptome patterns in 4T1 subclones with different expression levels of Ruk/CIN85 correlated with their ability to form mammospheres.

Keywords: adaptor protein Ruk/CIN85, breast adenocarcinoma, cancer stem cells, reprogramming, spheroids
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