Changes in the levels of vitamin D receptor and active form of the nuclear factor κB in bone tissue of rats with experimental type 1 diabetes mellitus and their correction with cholecalciferol

1Mazanova, AO, 1Shymanskyi, IO, 1Lisakovska, OO, 1Vasylevska, VM, 1Lototska, OYu., 1Makarova, OO, 1Veliky, MM
1O. V. Palladin Institute of Biochemistry of the NAS of Ukraine, Kyiv
Dopov. Nac. akad. nauk Ukr. 2018, 2:109-116
https://doi.org/10.15407/dopovidi2018.02.109
Section: Biochemistry
Language: Ukrainian
Abstract: 

It has been shown that chronic hyperglycemia, caused by the development of experimental type 1 diabetes mellitus (DM 1), leads to a significant decrease in the blood serum level of 25OHD. Vitamin D3 deficiency in rats with DM 1 is accompanied by the impaired signaling of calcitriol in bone tissue, as is evident from a decrease in the expression of the vitamin D3 receptor protein (VDR). The elevated level of nuclear factor κB (NF-κB) subunit p65 phosphorylated at Ser 311 in bone tissue is found. This may contribute to an increase in osteoclastogenesis. Supplementation of cholcalciferol (vitamin D3) to rats with DM 1 leads to a normalization of 25OHD in blood serum, which can result in the restoration of the osteoblastic-osteoclastic balance in bone tissue.

Keywords: nuclear factor-κB, osteoporosis, type 1 diabetes mellitus, vitamin D receptor, vitamin D3 (cholecalciferol)
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