Plant cells formed their protective structures use molecules of phytopathogenic microorganisms

1Emelyanov, VI, 1Polyakovskiy, SA, 1Sakada, VI, 1Grodzinskiy, DM
1Institute of Cell Biology and Genetic Engineering of the NAS of Ukraine, Kyiv
Dopov. Nac. akad. nauk Ukr. 2018, 3:110-115
Section: Biology
Language: Russian

During pathogenesis, plant cells are capable to forming their own structures at the expense of the molecules, which were a part of fungi cell walls. Autoradiographic analysis showed the presence of 14C-glucose in cells of onion, which were recently the compounds of the cell walls Botrytis senerea. Radioisotope method confirmed the presence of labeled glucose in Allium cera cells, the protective poly-β-1,3-glucose – callose.

Keywords: Allium sera, Botrytis senerea, callose, phytopathogenic microorganisms, plant cells
  1. Emelyanov, V. I. & Dmitriev, A. P. (2007). Induced increase in chitinase activity in tomato cells (Lycopersicon esculentum L.). Cytol. Gen., 41, Iss. 5, pp. 284-287. doi:
  2. Benhamou, N., Joosten, M., De Wit, P. J. G. M. (1990). Subcellular localization of chitinase and of its potential substrate in tomato root tissue infected by Fusarium oxysporum f. sp. radicis-lycopersici. Plant Physiol., 92, pp. 1108-1120. doi:
  3. Baureithel, K., Felix, G. & Boller, T. (1994). Specific, high affinity binding of chitin fragments to tomato cells and membranes: Competitive inhibition of binding by derivatives of chitooligosaccharides and a Nod factor of Rhizobium. J. Biol. Chem., 27, pp. 17931-17938.
  4. Tarchevsky, I.A. (2002). Plant cell signaling systems. Moscow: Nauka (in Russian).
  5. Emelyanov, V. I., Kravchuk, J. N., Polyakovskiy, S. A. & Dmytriev, A. P. (2008). Callose accumulation under treatment of tomato (Lycopersicon esculentum L.) cells with biotic elicitors. Tsitol. Genet., 42, No 2, pp. 21-28 (in Russian).
  6. Emelyanov, V.I. (2010). Calcium-independent mechanism of callose synthesis in plant cell. Dopov. Nac. akad. nauk Ukr., No 7, pp. 146-148 (in Russian).
  7. Nishimura, M. T., Stein, M., Hou, B. H., Vogel, J. P., Edwards, H. & Somerville, S. (2003). Loss of callose synthase result in salicylic acid-dependent disease resistance. Science, 301, pp. 969-972. doi:
  8. Emelyanov, V. I. & Kravchuk, J. N. (2001). Callose induse accumulation in suspension cultures of onion and tomato. Visnyk Dnipropetr. un-tu. Biol. Ecol., 9, pp. 235-241 (in Russian).
  9. Kauss, H. (1996). Callose synthesis. In Membranes: Specialized functions in plants (pp. 77-92). Guildford: Bios Scientific Publishers.
  10. Polyakovskiy, S. A., Kravchuk, J. N. & Dmytriev, A. P. The mechanisms of action of plant resistance inductors by the example of Allium cepa. Tsitol. Genet., 42, No. 6, pp. 8-12 (in Russian).
  11. Emelyanov, V. I., Dmytriev, A. P. & Grodzinskiy, D. M. (1999). Induction of chitinase activity by chitin fragments of different lengths in suspending culture cells of tomato (Lycopersicon esculentum). Dopov. Nac. akad. nauk Ukr., No 11, pp. 156-158 (in Ukrainian).
  12. Kondepudi, D. & Prigogine, I. (2002). Modern thermodynamics. From heat engines to dissipative structures. New York: Wiley.
  13. Dmytriev, A. P. (1999). Phytoalexins and their role in plant resistance. Kiev: Naukova Dumka (in Russian).
  14. Neduha, O. M. (2015). Callose: Localization, functions, and synthesis in plant cells. Cytol. Gen., 49, Iss. 1, pp. 49-57. doi: