Influence of human placenta extracts to erythrocytes' thermohemolysis

1Bobrova, OM, 1Govorova, Yu.S, 2Nardid, OA, 1Repina, SV, Narozhnyi, SV, Pokryshko, SV
1Institute for Problems of Cryobiology and Cryomedicine of the NAS of Ukraine, Kharkiv
2Institute for Problems of Cryobiology and Cryomedicine of the NAS of Ukraine, Kharkiv; V. N. Karazin Kharkiv National University
Dopov. Nac. akad. nauk Ukr. 2018, 7:82-87
https://doi.org/10.15407/dopovidi2018.07.082
Section: Biology
Language: Ukrainian
Abstract: 

The influence of the freezing of human placenta extracts on their ability to reduce the thermohemolysis of erythrocytes has been investigated. It has been shown that the freezing of placenta extracts down to −2 and −19 °C and the following heating do not lead to a significant change in their effectiveness to stabilize the erythrocyte membranes at temperature effects. The placenta extract fractions, namely less than 4 kDa and 12—20 kDa, most effectively reducing the level of erythrocytes' thermohemolysis, have been identified. The percentages of inhibition of the thermogemolysis for them are (31.7 ± 3.3) % and (31.5 ± 3.2) %, respectively.

Keywords: antiinflammatory efficacy, erythrocytes' thermohemolysis, fraction, freezing, placenta extract
References: 
  1. Grischenko, V. I. & Yurchenko, T. N. (Eds.). (2011). Placenta: cryopreservation, structure, properties, prospects of clinical use. Kharkiv (in Russian).
  2. Tiwary, S. K., Shukla, D., Tripathi, A.K., Agrawal, S., Singh, M. K. & Shukla, V. K. (2006). Effect of placentalextract gel and cream on healing wounds. J. Wound Care, 15, No. 7, pp. 325-328. doi: https://doi.org/10.12968/jowc.2006.15.7.26937
  3. De, D., Chakraborty, P. D. & Bhattacharyya, D. (2011). Regulation of trypsin activity by peptide fraction of aqueous extract of human placenta used as wound healer. J. Cell Physiol., 226, No. 8, pp. 2033-2040. doi: https://doi.org/10.1002/jcp.22535
  4. Bhadoriya, S. S., Mishra, V., Raut, S., Ganeshpurkar, A. & Jain, S. K. (2012). Anti-Inflammatory and antinociceptive activities of a hydroethanolic extract of tamarindus indica leaves. Sci. Pharm., 80, No. 3, pp. 685-700. doi: https://doi.org/10.3797/scipharm.1110-09
  5. Okoli, C. O., Akah, P. A, Onuoha, N. J., Okoye, Th. C., Nwoye, A. C. & Nworu, Ch. S. (2008). Acanthus montanus: An experimental evaluation of the antimicrobial, anti-inflammatory and immunological properties of a traditional remedy for furuncles. BMC Compl. Alternative Med., 8: 27. doi: https://doi.org/10.1186/1472-6882-8-2
  6. Bougandoura, A., D'Abrosca, B., Ameddah, S., Scognamiglio, M., Mekkiou, R., Fiorentino, A., Benayache, S. & Benayache, F. (2016). Chemical constituents and in vitro anti-inflammatory activity of Cistanche violacea Desf. (Orobanchaceae) extract. Fitoterapia, 109, No. 2, pp. 248-253. doi: https://doi.org/10.1016/j.fitote.2016.01.010
  7. Banerjee, K. K., Bishayee, A. & Chatterjee, M. (1994). Role of human placental extract on succinic dehydrogenase activity in carrageenin-induced edema in rats in vivo and its effect on erythrocyte lysis, platelet aggregation and trypsin activity in vitro. Indian J. Physiol. Pharmacol., 38, No. 2, pp. 121-124. PMID: 8063356
  8. Banerjee, K. K., Bishayee, A. & Chatterjee, M. (1992). Anti-inflammatory effect of human placental extract: a biochemical mechanistic approach. Riv. Eur. Sci. Med. Farmacol., 14, No. 6, pp. 361-366. PMID: 1308603
  9. Sharma, K. & Bhattacharyya, D. (2015). Immunoglobulin isotype isolated from human placental extract does not interfere in complement-mediated bacterial opsonization within the wound milieu. FEBS Open Bio, 5, pp. 369-377. doi: https://doi.org/10.1016/j.fob.2015.04.007
  10. Anosike, Ch. A., Obidoa, On. & Ezeanyika, L. (2012). US Membrane stabilization as a mechanism of the antiinflammatory activity of methanol extract of garden egg (Solanum aethiopicum). DARU, 20: 76. doi: https://doi.org/10.1186/2008-2231-20-76
  11. Ivanov, I. T., Brähler, M., Georgieva, R. & Bäumler, H. (2007). Role of membrane proteins in thermal damage and necrosis of red blood cells. Thermochim. Acta, 456, pp. 7-12. doi: https://doi.org/10.1016/j.tca.2007.01.020