Peculiarities of the growth and the content of endogenous cytokinins in the mycelial biomass of basidial mushrooms Hericium coralloides and Fomitopsis officinalis growing in vitro

1Vedenicheva, NP
Al-Maali, GA
Bisko, NA
1Shcherbatiuk, MM
1Kosakivska, IV
1M. G. Kholodny Institute of Botany of the NAS of Ukraine, Kyiv
Dopov. Nac. akad. nauk Ukr. 2018, 9:97-104
Section: Biology
Language: Russian

Growth dynamics and features of the accumulation of endogenous cytokinins in the mycelial biomass of medicinal basidial mushrooms Hericium coralloides and Fomitopsis officinalis at the various stages of cultivation in vitro have been analyzed. Accumulation dynamics of individual cytokinin forms is found to be species-specific. The maximum cytokinin content in H. coralloides occurred in the phase of an intensive growth, and the growth slowed down along with a gradual decrease in their levels. In F. officinalis, no clear correlation between the mycelial biomass growth and the accumulation of cytokinins was observed. Changes in the endogenous cytokinins accumulation pattern detected during the mycelial biomass growth are considered as an indirect evidence of the involvement of these phytohormones in the regulation of the growth and development of basidial mushrooms.

Keywords: Basidiomycetes, cytokinins, growth, mycelial biomass
  1. Zürcher, E. & Müller, B. (2016). Cytokinin synthesis, signaling and function — advances and new insights. Int. Rev. Cell Mol. Biol., 324, pp. 1-38. doi:
  2. Frébort, I., Kowalska, M., Hluska, T., Frébortová, J. & Galuszka, P. (2011). Evolution of cytokinin biosynthesis and degradation. J. Exp. Bot., 62, No 8, pp. 2431-2452. doi:
  3. Chanclud, E. & Morel, J.-B. (2016). Plant hormones: a fungal point of view. Mol. Plant Pathol., 17, No 8, pp. 1289-1297. doi:
  4. Chatterjee, S., Chatterjee, B. P. & Guha, A. K. (2008). Enhancement of growth and chitosan production by Rhizopus oryzae in whey medium by plant growth hormones. Int. J. Biol. Macromol., 42, No. 2, pp. 120-126. doi:
  5. Safaei, Z., Karimi, K., Golkar, P. & Zamani, A. (2015). Effects of plant hormones on Mucor indicus growth and chitosan and ethanol production. Int. J. Mol. Sci., 16, No. 7, pp. 16683-16694. doi:
  6. Ramachela, K., Sihlangu, S. M. & Moral, M. T. (2016). Effect of various hormonal treated plant substrates on development and yield of Pleurotus ostreatus. Cogent Food Agricult., 2, No. 1, 1276510.
  7. Mukhopadhyay, R., Chatterjee, S., Chatterjee, B. P. & Guha, A. K. (2005). Enhancement of biomass production of edible mushroom Pleurotus sajor-caju grown in whey by plant growth hormones. Proc. Biochem., 40, No. 3-4, pp. 1241-1244. doi:
  8. Guha, A. K. & Banerjee, A. B. (1974). Effect of indole-3-acetic acid and kinetin on submerged growth of Agaricus campestris. Acta Microbiol. Pol., 6, No. 3, pp. 133-134.
  9. Türker, M., Demirel, K., Uzun, Y., Battal, P. & Tileklioǧlu, B. (2005). Determination of phytohormones level in some dried and fresh macrofungi taxa. Phyton-Ann. Rei Bot., 45, pp. 145-157.
  10. Morrison, E. N., Knowles, S., Hayward, A., Thorn, R. G., Saville, B. J. & Emery, R. J. (2015). Detection of phytohormones in temperate forest fungi predicts consistent abscisic acid production and a common pathway for cytokinin biosynthesis. Mycologia, 107, No. 2, pp. 245-257. doi:
  11. Bisko, N. A., Lomberg, M. L., Mytropolska, N. Yu. & Mykchaylova, O. B. (2016). The IBK Mushroom Culture Collection. Kiev: Alterpres.
  12. Vedenicheva, N. P., Al-Maali, G. A., Mytropolska, N. Yu., Mykhaylova, O. B., Bisko, N. A. & Kosakivska, I. V. (2016). Endogenous cytokinins in medicinal basidiomycetes mycelial biomass. Biotechnol. Acta, 9, No. 1, pp. 55-63. doi:
  13. Vedenicheva, N. P. & Kosakivska, I. V. (2017). Cytokinins as regulators of plant ontogenesis under different growth conditions. Kiev: Nash Format (in Ukrainian).
  14. Özcan, B. (2001). GA3, ABA and cytokinin production by Lentinus tigrinus and Laetiporus sulphureus fungi cultured in the medium of olive oil mill waste. Turk. J. Biol., 25, No. 4, pp. 453-462.
  15. Grant, M. R. & Jones, J. D. (2009). Hormone (dis)harmony moulds plant health and disease. Science, 324(5928), pp. 750-752. doi: