Properties of the low molecular weight carotovoricins from the natural isolates Pectobacterium carotovorum subsp. carotovorum

1Maksymenko, LA
Gorb, TY
1D.K. Zabolotny Institute of Microbiology and Virology of the NAS of Ukraine, Kyiv
Dopov. Nac. akad. nauk Ukr. 2019, 6:67-74
https://doi.org/10.15407/dopovidi2019.06.067
Section: Biology
Language: Ukrainian
Abstract: 

Colicin-like carotovoricins (CCTV) has been induced by the nalidixic acid treatment of Pectobacterium carotovorum subsp. carotovorum natural isolates from the different regions of Ukraine. It has been found that CCTV exhibit the different level of killer activity against the indicator strain of P. carotovorum and Escherichia coli. CCTV isolated from total fractions of P. carotovorum J2 and natural bacterial isolates from different regions of Ukraine had the same set of proteins with molecular masses of 18, 20, 24, 30, 46, and 54 kDa. After the purification carried out at DEAE-sepharose, the active components with molecular masses of 18, 24, 30, and 38 kDa were isolated. Immunoblot analysis with antiserum revealed serologically related protein components with molecular masses of 10, 11, 18, and 20 kDa in low molecular weight carotovoricins and bacteriophage ZF 40. It has been established that cartovoricins also contain proteins that are not identical, but serologically related to ZF40.

Keywords: colicin-like carotovoricins (CCTV), killer activity, Pectobacterium carotovorum subsp. carotovorum, proteins, serological similarity
References: 

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