Peculiarities of the radiation-induced bystander effect manifestation in human peripheral blood lymphocytes due to action of astaxantine

1Rushkovsky, SR
2Kurinnyi, DA
Demchenko, OM
2Pilinska, MA
1Institute of Biology and Medicine, Taras Shevchenko National University of Kyiv
2National Research Center for Radiation Medicine of the NAMS of Ukraine, Kyiv
Dopov. Nac. akad. nauk Ukr. 2019, 9:82-87
https://doi.org/10.15407/dopovidi2019.09.082
Section: Biochemistry
Language: Ukrainian
Abstract: 

Using the method of Comet assay under neutral conditions, the effect of astaxanthin on the manifestation of the bystander effect is studied. Intact human lymphocytes were cocultivated with lymphocytes γ-irradiated in vitro in a dose 0.5 Gy. A considerable decrease in the DNA exit in cultures of bystander cells compared with the control cultures is shown. This phenomenon can be explained by the existence, in the culture of bystander lymphocyte, a significant number of damaged cells, in which the checkpoint on the S phase of the cell cycle is activated. Аstaxanthin had an influence on the realization of the bystander effect by reducing the number of cells, which obviously stopped their division in the S phase of the cell cycle, and increasing the frequency of cells with high level of DNA fragmentation as well.

Keywords: astaxanthine, bystander effect, Comet assay, human peripheral blood lymphocytes, γ-irradiation
References: 

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