Immunological reactions of rats on transplantation of microencapsulated tissue of human thyroid gland

1Pasteur, IP
1Baranova, LA
2Dmytrukha, NM
2Lahutina, OS
1V.P. Komisarenko Institute of Endocrinology and Metabolism of the NAMS of Ukraine, Kyiv
2Institute for Occupational Health of the NAMS of Ukraine, Kyiv
Dopov. Nac. akad. nauk Ukr. 2020, 1:95-102
https://doi.org/10.15407/dopovidi2020.01.095
Section: Medicine
Language: Ukrainian
Abstract: 

One of the methods for treating the endocrine gland hypofunction is a transplantation of appropriate tissues or cells that are microencapsulated in alginate capsules. The aim of the work was to investigate the safety of the method for immunological parameters. Microencapsulation and transplantation of human thyroid gland tissue in rats intraperitoneally, as well as immunological studies, were carried out according to standard methods. Reliable changes in the number of leukocytes and monocytes, the bactericidal activity of blood neutrophils, complement titer, the MTT test value of peritoneal macrophages, as well as the phagocytic activity of blood neutrophils and the number of macrophages in peritoneal exudate are established. These data indicate the need for the additional purification of a commercial biopolymer before its use in animal experiments.

Keywords: human thyroid gland tissue, immunological reactions, microencapsulation, rats, transplantation
References: 

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