Biological and physicochemical properties of cymbidium mosaic virus

1Parkhomenko, NI
2Maksymenko, LA
2Didenko, LF
1Institute of Macromolecular Chemistry of the NAS of Ukraine, Kyiv
2D.K. Zabolotny Institute of Microbiology and Virology of the NAS of Ukraine, Kyiv
Dopov. Nac. akad. nauk Ukr. 2020, 10:71-76
https://doi.org/10.15407/dopovidi2020.10.071
Section: Biology
Language: Ukrainian
Abstract: 

The isolated cymbidium mosaic virus (CMV) is one of the most wide-spread and dangerous pathogens that infects promising varieties of orchids. It causes characteristic symptoms on orchid plants, which are manifested in the form of a mosaic. Over time, these areas are necrotized, leading to the stop of flowering the plants and reducing their decorative value. The CyMV is not spread by insects-carriers, but is transmitted by the mechanical inoculation with juice. Electron microscopy revealed flexible filamentous viral particles with a length of about 500 nm. The purified viral preparation is sedimented with a single peak with a sedimentation coefficient of 142S. The floating density of the virus in the preformed CsCl gradient corresponded to 1.3 g/cm3. The electrophoretic analysis of proteins in polyacrylamide gel under denatured conditions showed the presence of two polypeptides with molecular weights of 27 and 31 kDa. RNA CyMV has a molecular weight of 2 · 106 Da. In the translation system of rabbit reticulocytes in vitro, a protein with a molecular weight of about 27 kDa is synthesized. The obtained data allow us to refer CyMV to the group of potexviruses.

Keywords: cymbidium mosaic virus, orchids, polypeptides, RNA
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