Enhancement of erythrocyte oxygen transport function after hydrothermic storage under the action of human cord blood low-molecular fraction and the Actovegin drug

1Gulevskyy, OK
1Akhatova, Yu.S
Zharkova, YY
1Institute for Problems of Cryobiology and Cryomedicine of the NAS of Ukraine, Kharkiv
Dopov. Nac. akad. nauk Ukr. 2020, 11:85-91
https://doi.org/10.15407/dopovidi2020.11.085
Section: Biochemistry
Language: Ukrainian
Abstract: 

We study the effect of a low-molecular fraction (below 10 kDa) derived from human cord blood and Actovegin on the content of various forms of hemoglobin in erythrocytes stored under hypothermia (2-4 ºС) for 21 days. During the long-term storage of erythrocytes, there is known to be a change in the hemoglobin forms ratio toward the decrease of the relative content of oxyhemoglobin and an increase of deoxy- and methemoglobin. As a result, the affinity of hemoglobin for oxygen reduces. The incubation of erythrocytes in a rehabilitation medium with each of the studied low-molecular drugs was found to be crucial to increase the content of oxyhemoglobin and to reduce the amounts of deoxy- and methemoglobin relative to the control. As a result, there was a rise in the oxygenation coefficient, reflecting the oxygen saturation of hemoglobin, up to the level characteristic of freshly isolated erythrocytes. The data obtained show that the human cord blood low-molecular fraction and the Actovegin normalize the ratio of hemoglobin forms in hypothermically stored erythrocytes, but the use of the Actovegin drug is more effective.

Keywords: Actovegin, cord blood, erythrocytes, hypothermia, oxygen transport function
References: 

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