|1Gulevskyy, OK |
1Institute for Problems of Cryobiology and Cryomedicine of the NAS of Ukraine, Kharkiv
|Dopov. Nac. akad. nauk Ukr. 2020, 11:85-91|
We study the effect of a low-molecular fraction (below 10 kDa) derived from human cord blood and Actovegin on the content of various forms of hemoglobin in erythrocytes stored under hypothermia (2-4 ºС) for 21 days. During the long-term storage of erythrocytes, there is known to be a change in the hemoglobin forms ratio toward the decrease of the relative content of oxyhemoglobin and an increase of deoxy- and methemoglobin. As a result, the affinity of hemoglobin for oxygen reduces. The incubation of erythrocytes in a rehabilitation medium with each of the studied low-molecular drugs was found to be crucial to increase the content of oxyhemoglobin and to reduce the amounts of deoxy- and methemoglobin relative to the control. As a result, there was a rise in the oxygenation coefficient, reflecting the oxygen saturation of hemoglobin, up to the level characteristic of freshly isolated erythrocytes. The data obtained show that the human cord blood low-molecular fraction and the Actovegin normalize the ratio of hemoglobin forms in hypothermically stored erythrocytes, but the use of the Actovegin drug is more effective.
|Keywords: Actovegin, cord blood, erythrocytes, hypothermia, oxygen transport function|
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