Participation of nitric oxide in induction of heat resistance of wheat seedlings by putrescine

Kokorev, AI
1Kolupaev, Yu.E
1Karpets, Yu.V
Dyachenko, AI
1V. V. Dokuchaev National Agrarian University of Kharkiv
Dopov. Nac. akad. nauk Ukr. 2020, 12:85-92
https://doi.org/10.15407/dopovidi2020.12.085
Section: Biology
Language: Ukrainian
Abstract: 

Polyamines are stress plant metabolites involved in the formation of adaptive responses. Recently, they have been considered as compounds that are involved in signaling processes. However, the possible role of nitric oxide (NO) and its functional interaction with reactive oxygen species (ROS) in the realization of stress-protective effects of polyamines has been very poorly studied. The participation of NO as a signaling mediator in the induction of the heat resistance of wheat seedlings by the exogenous diamine putrescine is studied. Incubation of intact seedling roots on a 1 mM putrescine solution caused an increase in the activity of diamine oxidase and a transient increase in the content of nitric oxide and hydrogen peroxide, respectively, with peaks at 1 and 2 hours from the start of the treatment. The increase in the NO content was eliminated by the action of aminoguanidine (an inhibitor of diamine oxidase and NO synthase), but not sodium tungstate (an inhibitor of nitrate reductase). Treatment of seedlings with a scavenger of hydrogen peroxide with dimethylthiourea (DMTU) reduced the effect of increasing the NO content in roots caused by putrescine. At the same time, scavenger NO PTIO (2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide) almost completely eliminated the accumulation of hydrogen peroxide in the roots, which occurred under the action of putrescine. The increase in the heat resistance of seedlings induced by the treatment with putrescine was completely eliminated under the influence of the NO PTIO scavenger, an inhibitor of diamine oxidase and NO synthase aminoguanidine, and the antioxidant DMTU. It is concluded about the functional interaction of ROS and NO as a signal intermediaries, providing the induction of protective responses and the development of plants of heat-resistance in the processing of putrescine.

Keywords: heat resistance, hydrogen peroxide, nitric oxide (NO), polyamines, putrescine, signaling mediators, Triticum aestivum.
References: 

1. Pal, M., Szalai, G. & Janda, T. (2015). Speculation: Polyamines are important in abiotic stress signaling. Plant Sci., 237, pp. 16-23. https://doi.org/10.1016/j.plantsci.2015.05.003
2. Asthir, B., Kumar, R. & Bains, N.S. (2018). Why and how putrescine modulates thermotolerance in wheat? Indian J. Biochem. Biophys., 55, No. 6, pp. 404-412. http://nopr.niscair.res.in/handle/123456789/45454
3. Scaramagli, S., Biondi, S., Leone, A., Grillo, S. & Torrigiani, P. (2000). Acclimation to low water potential in potato cell suspension cultures leads to changes in putrescine metabolism. Plant Physiol. Biochem., 38, No. 4, pp. 345-351. https://doi.org/10.1016/S0981-9428(00)00750-6
4. Li, Z., Zhang, Y., Zhang, X., Peng, Y., Merewitz, E., Ma, X., Linkai, H. & Yanhong, Y. (2016). The alterations of endogenous polyamines and phytohormones induced by exogenous application of spermidine regulate antioxidant metabolism, metallothionein and relevant genes conferring drought tolerance in white clover. Environ. Exp. Bot., 124, pp. 22-38. https://doi.org/10.1016/j.envexpbot.2015.12.004
5. Kuznetsov, Vl. V., Radyukina, N. L. & Shevyakova, N. I. (2006). Polyamines and stress: Biological role, metabolism, and regulation. Russ. J. Plant Physiol., 53, No. 5, pp. 583-604. https://doi.org/10.1134/S1021443706050025
6. Kolupaev, Yu. E., Karpets, Yu. V. & Dmitriev, A. P. (2015). Signal mediators in plants in response to abiotic stress: calcium, reactive oxygen and nitrogen species. Cytol. Genet., 49, No. 5, pp. 338-348. https://doi.org/10.3103/S0095452715050047
7. Yang, B., Wu, J., Gao, F., Wang, J. & Su, G. (2014). Polyamine-induced nitric oxide generation and its po tential requirement for peroxide in suspension cells of soybean cotyledon node callus. Plant Physiol. Biochem., 79, pp. 41-47. https://doi.org/10.1016/j.plaphy.2014.02.025
8. Wimalasekera, R., Villar, C., Beguma, T. & Scherer, G.F.E. (2011). COPPER AMINE OXIDASE1 (CuAO1) of Arabidopsis thaliana contributes to abscisic acid- and polyamine-induced nitric oxide biosynthesis and abscisic acid signal transduction. Mol. Plant., 4, No. 4, pp. 663-678. https://doi.org/10.1093/mp/ssr023
9. Wimalasekera, R., Tebartz, F. & Scherer, G.F.E. (2011). Polyamines, polyamine oxidases and nitric oxide in development, abiotic and biotic stresses. Plant Sci., 181, No. 5, pp. 593-603. https://doi.org/10.1016/j.plantsci.2011.04.002
10. Kolupaev, Yu.E., Kokorev, A.I., Yastreb, T.O. & Horielova, E.I. (2019). Hydrogen peroxide as a signal mediator at inducing heat resistance in wheat seedlings by putrescine. Ukr. Biochem. J., 91, No. 6, pp. 103-111. https://doi.org/10.15407/ubj91.06.103
11. Naik, B.I., Goswami, R.G. & Srivastava, S.K. (1981). A rapid and sensitive calorimetric assay of amine oxidase. Anal. Biochem., 111, No. 1, pp. 146-148. https://doi.org/10.1016/0003-2697(81)90242-6
12. Karpets, Yu.V., Kolupaev, Yu.E. & Vayner, A.A. (2015). Functional interaction between nitric oxide and hydrogen peroxide during formation of wheat seedling induced heat resistance. Russ. J. Plant Physiol., 62, No. 1, pp. 65-70. https://doi.org/10.1134/S1021443714060090
13. Sagisaka, S. (1976). The occurrence of peroxide in a perennial plant, Populus gelrica. Plant Physiol., 57, No. 2, pp. 308-309. https://doi.org/10.1104/pp.57.2.308
14. Li, Z., Zhu, Y., He, X., Yong, B., Peng, Y., Zhang, X., Ma, X., Yan, Y., Huang, L. & Nie, G. (2019).The hydrogen sulfide, a downstream signaling molecule of hydrogen peroxide and nitric oxide, involves spermidi neregulated transcription factors and antioxidant defense in white clover in response to dehydration. Envi ron. Exp. Bot., 161, pp. 255-264. https://doi.org/10.1016/j.envexpbot.2018.06.036
15. Kolbert, Z., Barroso, J.B., Brouquisse, R., Corpas, F.J., Gupta, K.J., Lindermayr, C., Loake, G.J., Palma, J.M., Petřivalský, M., Wendehenne, D. & Hancock, J.T. (2019). A forty year journey: The generation and roles of NO in plants. Nitric Oxide, 93, pp. 53-70. https://doi.org/10.1016/j.niox.2019.09.006