Individual features of radiation-induced genomic instability in patients with glioblastoma

Zemskova, OV
Glavatsky, OY
1Kurinnyi, DA
Demchenko, OM
2Rushkovsky, SR
1National Research Center for Radiation Medicine of the NAMS of Ukraine, Kyiv
2Institute of Biology and Medicine, Taras Shevchenko National University of Kyiv
Dopov. Nac. akad. nauk Ukr. 2020, 4:91-98
https://doi.org/10.15407/dopovidi2020.04.091
Section: Biology
Language: Ukrainian
Abstract: 

Using the method of Comet assay under neutral conditions, the features of individual radiation-induced genome instability in patients with glioblastoma was studied. It was found that, in the culture of peripheral blood lymphocytes of two patients with pathomorphologically verified glioblastoma (patients No. 1 and No. 3), the frequency of comets with a high level of DNA damage significantly exceeded the values in the cultures of blood lymphocytes of the comparison group. After irradiation in a dose of 1.0 Gy, the frequency of cells with a high DNA damage rate was increased in lymphocyte cultures of patients No. 2 and No. 3 and decreased in patient No. 1. Frequency analysis of the distribution of individual “comets” depending on their levels of DNA damage revealed the presence of a significant pool of cells in the lymphocyte cultures of patients No. 2 and No. 3 which stopped di vision at the S stage of the cell cycle. After the irradiation, the frequency of such cells in patient No. 3 decreased significantly. It was noted that apoptotic activity in cultures of lymphocytes of neuro-oncological patients was significantly higher than in cultures of conditionally healthy volunteers.

Keywords: apoptosis, Comet assay, glioblastoma, human peripheral blood lymphocytes culture, γ-irradiation
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