|Zemskova, OV |
1National Research Center for Radiation Medicine of the NAMS of Ukraine, Kyiv
2Institute of Biology and Medicine, Taras Shevchenko National University of Kyiv
|Dopov. Nac. akad. nauk Ukr. 2020, 4:91-98|
Using the method of Comet assay under neutral conditions, the features of individual radiation-induced genome instability in patients with glioblastoma was studied. It was found that, in the culture of peripheral blood lymphocytes of two patients with pathomorphologically verified glioblastoma (patients No. 1 and No. 3), the frequency of comets with a high level of DNA damage significantly exceeded the values in the cultures of blood lymphocytes of the comparison group. After irradiation in a dose of 1.0 Gy, the frequency of cells with a high DNA damage rate was increased in lymphocyte cultures of patients No. 2 and No. 3 and decreased in patient No. 1. Frequency analysis of the distribution of individual “comets” depending on their levels of DNA damage revealed the presence of a significant pool of cells in the lymphocyte cultures of patients No. 2 and No. 3 which stopped di vision at the S stage of the cell cycle. After the irradiation, the frequency of such cells in patient No. 3 decreased significantly. It was noted that apoptotic activity in cultures of lymphocytes of neuro-oncological patients was significantly higher than in cultures of conditionally healthy volunteers.
|Keywords: apoptosis, Comet assay, glioblastoma, human peripheral blood lymphocytes culture, γ-irradiation|
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