Temperature, osmolality, and glucose determine the erythrocyte resistance to post-hypertonic stress

Semionova, KA
Nipot, OE
Yershova, NA
Shapkina, OО
1Shpakova, NM
1Institute for Problems of Cryobiology and Cryomedicine of the NAS of Ukraine, Kharkiv
Dopov. Nac. akad. nauk Ukr. 2020, 4:99-106
https://doi.org/10.15407/dopovidi2020.04.099
Section: Biology
Language: Ukrainian
Abstract: 

The effect of glucose on the sensitivity of human and rabbit erythrocytes to post-hypertonic shock has been investigated. The level of post-hypertonic hemolysis of human and rabbit erythrocytes was found to depend on the dehydration medium and to increase with a rise in the salt concentration in it. An analysis of the sensitivity of these cells to the effects of post-hypertonic shock showed that rabbit erythrocytes were more resistant, i.e. at 37 °C, the injury rate of rabbit erythrocytes was at least a half lower of that for human cells; at 0 °C, the effect was less pronounced. The level of post-hypertonic hemolysis of the erythrocytes pretreated with glucose was determined by endogenous (species belonging to erythrocytes) and exogenous factors (modifier concentration and post-hypertonic shock conditions). In case of human erythrocytes, glucose was capable of reducing the post-hypertonic hemolysis, which exceeded 50 %. If, under conditions of post-hypertonic shock, glucose in a high concentration (5 %) was effective at 37 and 0 °C, then, in low (0.6 %) one, it was effective only at 0 °C. In rabbit erythrocytes, glucose was capable of reducing a post-hypertonic hemolysis only when used at a high concentration (5 %) in post-hypertonic shock at 37 °C. Glucose is thought to form hydrogen bonds with cytoplasmic proteins, which impedes the binding of sodium ions to them. This prevents the excess flow of ions into a cell during the dehydration step and, accordingly, the development of erythrocyte injury during rehydration. It is envisaged that the adherence to near-zero temperatures when thawing human erythrocytes and removing cryoprotectants will allow the use of lower concentrations of glucose to achieve a protective effect and prevent its toxic effect on cells.

Keywords: dehydration, erythrocyte, glucose, post-hypertonic shock, rehydration, temperature
References: 

1. Cancelas, J. A., Dumont, L. J., Maes, L. A., Rugg, Herschel, N. L., Whitley, P. H., Szczepiokowski, Z. M., Siegel, A. H., Hess, J. R. & Zia, M. (2015). Additive solution-7 reduces the red blood cell cold storage lesion. Transfusion, 55, No. 3, pp. 491-498. Doi: https://doi.org/10.1111/trf.12867
2. Quan, G. B., Han, Y., Liu, M. X., Fang, L., Du, W., Ren, S. P., Wang, J. X. & Wang, Y. (2011). Addition of oligosaccharide decreases the freezing lesions on human red blood cell membrane in the presence of dextran and glucose. Cryobiology, 62, No. 2, pp. 135-144. Doi: https://doi.org/10.1016/j.cryobiol.2011.01.015
3. Elliott, G. D., Wang, S. & Fuller, B. J. (2017). Cryoprotectants: A review of the actions and applications of cryoprotective solutes that modulate cell recovery from ultra-low temperatures. Cryobiology, 76, pp. 74-91. Doi: https://doi.org/10.1016/j.cryobiol.2017.04.004
4. Jain, S. K. (1989). Hyperglycemia can cause membrane lipid peroxidation and osmotic fragility in human red blood cells. J. Biol. Chem., 264, No. 35, pp. 21340-21345.
5. Resmi, H., Pekcetin, C. & Guner, G. (2001). Erythrocyte membrane and cytoskeletal protein glycation and oxidation in short-term diabetic rabbits. Clin. Exp. Med., No. 4, pp. 187-193. Doi: https://doi.org/10.1007/s102380100002
6. Vrhovac I., Breljak, D. & Sabolic, I. (2014). Glucose transporters in the mammalian blood cells. Period. Biol., 116, No. 2, pp. 131-138.
7. Shpakova, N. M. (2014). Temperature and osmotic resistance of erythrocytes of different mammalian species. (Extended abstract of Doctor thesis). Institute for Problems of Cryobiology and Cryomedicine of the NAS of Ukraine, Kharkiv, Ukraine (in Ukrainian).
8. Semionova, Ye. A., Yershova, N. A., Yershov, S. S., Orlova, N. V. & Shpakova, N. M. (2016). Peculiarities of posthypertonic lysis in erythrocytes of several mammals. Probl. Cryobiol. Cryomed., 26, No. 1, pp. 73-83 (in Russian). Doi: https://doi.org/10.15407/cryo26.01.073
9. Muldrew, K. (2008). The salting-in hypothesis of post-hypertonic lysis. Cryobiology, 57, No. 3, pp. 251-256. Doi: https://doi.org/10.1016/j.cryobiol.2008.09.007
10. Mensinka, M. A., Frijlinka, H. W., Maarschalk, K. V. & Hinrichsa, W. L. J. (2017). How sugars protect proteins in the solid state and during drying (review): Mechanisms of stabilization in relation to stress conditions. Eur. J. Pharm. Biopharm., 114, pp. 288-295. Doi: https://doi.org/10.1016/j.ejpb.2017.01.024
11. Eroglu, A., Russo, M. J., Bieganski, R., Fowler, A., Cheley, S., Bayley, H. & Toner, M. (2000). Intracellular trehalose improves the survival of frozen mammalian cells. Nat. Biotechnol., 18, No. 2, pp. 163-167. Doi: https://doi.org/10.1038/72608
12. Kanias, T. & Acker, J. P. (2009). Trehalose loading into red blood cells is accompanied with hemoglobin oxidation and membrane lipid peroxidation. Cryobiology, 58, No. 2, pp. 232-239. Doi: https://doi.org/10.1016/j.cryobiol.2008.12.003
13. Quan, G. B., Han, Y., Liu, M. X. & Gao, F. (2009). Effects of pre-freeze incubation of human red blood cells with various sugars on postthaw recovery when using a dextran-rapid cooling protocol. Cryobiology, 59, No. 3, pp. 258-267. Doi: https://doi.org/10.1016/j.cryobiol.2009.08.001
14. Wagner, R., Zimmer, G. & Lacko, L. (1984). An interspecies approach to the investigation of the red cell membrane glucose transporter. Biochim. Biophys. Acta, 771, No. 1, pp. 99-102. Doi: https://doi.org/10.1016/0005-2736(84)90115-9