|Semionova, KA |
1Institute for Problems of Cryobiology and Cryomedicine of the NAS of Ukraine, Kharkiv
|Dopov. Nac. akad. nauk Ukr. 2020, 4:99-106|
The effect of glucose on the sensitivity of human and rabbit erythrocytes to post-hypertonic shock has been investigated. The level of post-hypertonic hemolysis of human and rabbit erythrocytes was found to depend on the dehydration medium and to increase with a rise in the salt concentration in it. An analysis of the sensitivity of these cells to the effects of post-hypertonic shock showed that rabbit erythrocytes were more resistant, i.e. at 37 °C, the injury rate of rabbit erythrocytes was at least a half lower of that for human cells; at 0 °C, the effect was less pronounced. The level of post-hypertonic hemolysis of the erythrocytes pretreated with glucose was determined by endogenous (species belonging to erythrocytes) and exogenous factors (modifier concentration and post-hypertonic shock conditions). In case of human erythrocytes, glucose was capable of reducing the post-hypertonic hemolysis, which exceeded 50 %. If, under conditions of post-hypertonic shock, glucose in a high concentration (5 %) was effective at 37 and 0 °C, then, in low (0.6 %) one, it was effective only at 0 °C. In rabbit erythrocytes, glucose was capable of reducing a post-hypertonic hemolysis only when used at a high concentration (5 %) in post-hypertonic shock at 37 °C. Glucose is thought to form hydrogen bonds with cytoplasmic proteins, which impedes the binding of sodium ions to them. This prevents the excess flow of ions into a cell during the dehydration step and, accordingly, the development of erythrocyte injury during rehydration. It is envisaged that the adherence to near-zero temperatures when thawing human erythrocytes and removing cryoprotectants will allow the use of lower concentrations of glucose to achieve a protective effect and prevent its toxic effect on cells.
|Keywords: dehydration, erythrocyte, glucose, post-hypertonic shock, rehydration, temperature|
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