Tomato lines expressing human lactoferrin gene are characterized by enhanced resistance to late blight

Buziashvili, АY
Cherednichenko, LМ
Kropyvko, SV
Yemets, АІ
Dopov. Nac. akad. nauk Ukr. 2020, 5:95-102
Section: Biology
Language: Ukrainian

Transgenic lines of tomatoes (Lycopersicon esculentum Mill.) of cultivars Lahidny and Money Maker expressing human lactoferrin gene (hLf) were obtained with the use of Agrobacterium-mediated transformation. For the transfer of hLf gene, the pBin35LF plasmid vector was used with the gene-of-interest under control of 35 S promoter of cauliflower mosaic virus (CaMV35S) and the gene of neomycinephosphotransferase ІІ (nptII) conferring the resistance to kanamycin. Integration of hLf into genome of transgenic lines was confirmed with the use of PCR with specific primers to hLf. Expression of lactoferrin protein was detected with the use of Western blot analysis. The resistance of transgenic lines to Phytophthora infestans was studied in vitro with the use of the disk diffusion assay, infection of aseptic plants, and detached leaves. The enhanced resistance to late blight of transgenic tomato lines was shown as compared to control lines. The obtained results demonstrate that the expression of lactoferrin improve the resistance of transgenic tomato plants to aggressive fungal pathogens such as P. infestans.

Keywords: human lactoferrin gene, Lycopersicon esculentum Mill., Phytophthora infestans, resistance

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