Callusogenesis features of anthocyanin contrasting varieties of Lactuca sativa L

Pryimak, YV
Smirnov, OE
Taran, NY
Schwartau, VV
Dopov. Nac. akad. nauk Ukr. 2020, 7:94-100
Section: Biology
Language: Ukrainian

The selection of conditions for the callusogenesis introduction of two anthocyanin contrasting varieties of lettuce (Lactuca sativa L.) — Lolo Bionda (green) and Lolo Rossa (red) was approved. The effect of phytohormonal ratio, treatment with sterilizing agents on the induction of callusogenesis, calli area, consistency and structure of the six-week primary calli of both varieties were compared. Using media with a ratio of growth regulators: 6-benzylaminopurine (BAP) — 0.3 mg/L, naphthylacetic acid (NAA) — 2 mg/L, 2,4-dichlorophenoxyacetic acid (2,4-D) — 0.2 mg/L, and BAP — 10 mg/L, NAA — 0.5 mg/L led to callusogenesis stimulation from the cotyledon leaves explants of both varieties aseptic seedlings. A significant growth stimulation of primary Lolo Rossa calluses under sterilization of explants with mercury chloride and sodium hypochlorite was found. The heterogeneity of the cellular content of calls tissue and the possibility of obtaining loose and compact calluses of both varieties were investigated.

Keywords: callusogenesis, Lactuca sativa L., Lolo Bionda, Lolo Rossa, primary callus

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