Influence of carbon monoxide (CO) donor on heat resistance of wheat plantlets and generation of reactive oxygen species by them

Shkliarevskyi, MA
1Kolupaev, Yu.E
1Karpets, Yu.V
Shvidenko, MV
2Dmitriev, OP
1V. V. Dokuchaev National Agrarian University of Kharkiv
2Institute of Cell Biology and Genetic Engineering of the NAS of Ukraine, Kyiv
Dopov. Nac. akad. nauk Ukr. 2020, 8:73-80
https://doi.org/10.15407/dopovidi2020.08.073
Section: Biology
Language: Ukrainian
Abstract: 

The influence of hemin (donor of carbon monoxide CO formed due to action of heme oxygenase) on the heat resistance of wheat plantlets and the possible participation of reactive oxygen species (ROS) as mediators in the realization of stress-protective effects of exogenous CO have been studied. The treatment of plantlets with hemin in the concentrations of 0.5-5.0 μM caused a significant increase in their resistance to the damaging heating (10 min at a temperature of 45 °C). Under the action of hemin, the transient increase in the content of hydrogen peroxide in roots was observed with the maximum in 2 hours after the treatment start. In addition, the extracellular peroxidase activity increased temporarily. The treatment of roots with the inhibitor of peroxidase — sodium azide — eliminated both the increase in the activity of extracellular peroxidase and the rise in the content of hydrogen peroxide in roots caused by the action of hemin. At the same time, the treatment with the inhibitor of NADPH oxidase — imidazole — had almost no influence on the effect of an increase in the content of H2O2 under the action of hemin. Under the treatment of plantlets with a scavenger of hydrogen peroxide — dimethylthiourea — both the rise in the H2O2 content and the increase in the heat resistance after the action of hemin are not observed. All studied effects of hemin were eliminated under the influence of CO scavenger hemoglobin. The conclusion is made about the role of ROS, generated by extracellular peroxidase, as the mediators in the process of induction of the heat resistance of plantlets by the CO donor.

Keywords: carbon monoxide, heat resistance, hydrogen peroxide, peroxidase, signal mediators, Triticum aestivum
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