Title | Binding of mAb II-5c to Aα20–78 fragment of fibrinogen inhibits a neoantigenic determinant exposure within Bβ126–135 site of a molecule |
Publication Type | Journal Article |
Year of Publication | 2014 |
Authors | Urvant, LP, Makogonenko, EM, Pozniak, TA, Pydiura, NA, Kolesnikova, IN, Tsap, PY, Bereznitzkiy, GK, Lugovskoy, EV, Komisarenko, SV |
Abbreviated Key Title | Dopov. Nac. akad. nauk Ukr. |
DOI | 10.15407/dopovidi2014.05.149 |
Issue | 5 |
Section | Biochemistry |
Pagination | 149-156 |
Date Published | 5/2014 |
Language | Ukrainian |
Abstract | The influence of mAb II-5c, epitope of which was localized within Aα20–78 fragment of the E-region of fibrinogen molecule, on the exposure of mAb I-3c neoantigenic determinant at the transformation of fibrinogen to fibrin has been investigated. Using ELISA, SPR, and electrophoretic analysis, we have found that mAb II-5c inhibited the binding of mAb I-3c to fibrin, thrombin to fibrin, and thrombin cleavage of fibrinopeptide A from fibrinogen in fibrinogen + thrombin and X-fragment fibrinogen + thrombin systems. These data support our hypothesis that the thrombin-fibrinogen substrate complex is a trigger of the restructuring of a fibrinogen molecule at the transformation in fibrin, which is accompanied by the formation of neoantigenic determinants of mAb I-3c within Bβ126–135 site of a molecule. |
Keywords | Bβ126–135 site of a molecule, fibrinogen, mAb II-5c, neoantigenic determinant |
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