Stimulating effect of a low-molecular fraction from cord blood on the energy metabolism in leukocytes

TitleStimulating effect of a low-molecular fraction from cord blood on the energy metabolism in leukocytes
Publication TypeJournal Article
Year of Publication2014
AuthorsGulevsky, OK, Akhatova, Yu.S, Sysoev, AA, Sysoeva, IV
Abbreviated Key TitleDopov. Nac. akad. nauk Ukr.
Date Published7/2014

The ATP, ADP, and AMP contents are determined in leukoconcentrate cells after incubation in a medium containing a low-molecular fraction (below 5 kDa) from cord blood (0.15 mg/mL) by chemiluminescent analysis. It is shown that the fraction significantly stimulates the accumulation of intracellular ATP and ADP and has no effect on the AMP content. The stimulatory effect of the low-molecular fraction from cord blood is blocked by a glycolysis inhibitor, iodoacetate Na, and a glucose transport inhibitor, cytochalasin B. It is found that the fraction from cord blood enhances the total adenylate pool of leukocytes. It is established experimentally that the presence of the low-molecular fraction from cattle cord blood stimulates the glucose accumulation in leukoconcentrate cells.

Keywordscord blood, energy metabolism, leukocytes

1. Gulevskii A. K., Gorina O. L., Moiseeva N. N., Stepaniuk L. V. Ukr. zhurn. gematologii ta transfuzologii, 2010, No. 1: 22–29 (in Ukrainian).
2. Gulevskii A. K., Akhmatova Yu. S., Moiseeva N. N. et al. Probl. kriobiologii, 2012, 22, No. 1: 71–79 (in Russian).
3. Rumyantseva S. A. Actovegin. New aspects of clinical use. - Moscow: Izd-vo Mosk. un-ta, 2002. – 280 p.
4. Grishina V. V., Timokhina E. V., Andreeva L. Yu. Vopr. ginekologii, akusherstva i perinatologii, 2004, 3, No. 6: 50–54 (in Russian).
5. Pat. 69652 UA, IPC A61K35/14. Method for low-molecular fraction of cattle cord blood. Hulevskyi O. K., Moiseeva N. M., Abakumova O. S., Shcheniavskyi I. I., Nikolchenko A. Yu, Horina O. L. Publ. 10.05.2012 (in Ukrainian).
6. Medical imunobiolohichni drugs. Methodical recommendations for presentation to drugs technological rehlamentiv blood. Kyiv: Ministry of Health of Ukraine, 2005 (in Ukrainian).
7. Mayanskiy N. A. Immunologiia, 2004, No. 5: 307–312 (in Russian).
8. Wood T. E., Dalili S., Simpson C. D. et al. Mol. Cancer Ther., 2008, 7, No. 11: 3546–3555.
9. Pinegin B. V., Mayanskiy N. A. Immunologiia, 2007, No. 6: 374–382 (in Russian).
10. Gulevskii A. K., Veselovskaia Yu. S. Ukr. zhurn. gematologii ta transfuzologii, 2011, No. 6: 5–18 (in Ukrainian).
11. Scuster D. P., Brody S. L., Zhou Z. et al. Am. J. Phys. Lung Cell. Mol. Phys., 2007, 292, No. 4: 845–851.