Mechanisms of formation of targeted complex insertions under the synthesis of a DNA molecule containing cis-syn cyclobutane thymine dimers

TitleMechanisms of formation of targeted complex insertions under the synthesis of a DNA molecule containing cis-syn cyclobutane thymine dimers
Publication TypeJournal Article
Year of Publication2015
AuthorsGrebneva, HA
Abbreviated Key TitleDopov. Nac. akad. nauk Ukr.
DOI10.15407/dopovidi2015.05.144
Issue5
SectionBiology
Pagination144-153
Date Published5/2015
LanguageRussian
Abstract

The mechanism of formation of complex mutations has not been yet explained. Mutations are called complex if a DNA site with certain length and certain nucleotide composition is replaced by that with different length and different nucleotide composition. The author has proposed and developed a polymerase-tautomeric model of ultraviolet mutagenesis. The mechanism of formation of targeted complex insertions that are caused by cis-syn cyclobutane thymine dimers is proposed. The error-prone or SOS-synthesis of double-stranded DNA containing cis-syn cyclobutane thymine dimers TT*1, TT*2, and TT*5 in one of its strands is considered. Cis-syn cyclobutane thymine dimers TT*2 lead to frameshift mutations, i. e., to the insertions of one or more nucleotides. Cis-syn cyclobutane thymine dimers TT*1 and TT*5 cause the formation of substitution mutations. Thus, due to the formation of a DNA insertion, its portion is extended, and its nucleotide composition varies due to the formation of several base substitution mutations. As a result, a DNA portion with certain length and nucleotide composition is replaced by that with different length and different nucleotide composition. A targeted complex mutation appears.

Keywordscis-syn thymine cyclobutane dimers, error-prone replication, polymerase-tautomeric model, rare tautomeric forms of DNA bases, SOS replication, targeted complex insertions, targeted complex mutations, ultraviolet mutagenesis
References: 
  1. Wang C.-I., Taylor J.-S. Biochemistry, 1992, 31: 3671–3681. https://doi.org/10.1021/bi00129a016
  2. Abdulovic A. L., Jinks-Robertson S. Genetics, 2006, 172: 1487–1498. https://doi.org/10.1534/genetics.105.052480
  3. Levine J.G., Schaaper R.M., DeMarini D.M. Genetics, 1994, 136: 731–746.
  4. Horsfall M. J., Borden A., Lawrence C.W. J. Bacteriol., 1997, 179: 2835–2839. https://doi.org/10.1128/jb.179.9.2835-2839.1997
  5. Kunz B.A., Glickman B.W. Genetics, 1984, 106: 347–364.
  6. Grebneva H.A. Dopov. Nac. akad. nauk Ukr., 2001, No 7: 165–169 (in Russian).
  7. Grebneva H.A. J. Mol. Struct., 2003, 645: 133–143. https://doi.org/10.1016/S0022-2860(02)00578-1
  8. Grebneva H.A. Dopov. Nac. akad. nauk Ukr., 2001, No 8: 183–189 (in Russian).
  9. Grebneva H.A. Environ. Mol. Mutagen, 2006, 47: 733–745. https://doi.org/10.1002/em.20256
  10. Grebneva H.A. Molecular. Biology (Mosk.), 2014, 48: 457–467. https://doi.org/10.1134/S0026893314030066
  11. Grebneva H.A. Dopov. Nac. akad. nauk Ukr., 2014, No 11: 156–164 (in Russian).
  12. Grebneva H.A. Dopov. Nac. akad. nauk Ukr., 2013, No 1: 143–150 (in Russian).
  13. Grebneva H.A. Dopov. Nac. akad. nauk Ukr., 2012, No 10: 181–187 (in Russian).
  14. Raghunathan G., Kieber-Emmons T., Rein R., Alderfer J. L. J. Biomol. Struct. Dynam., 1990, 7: 899–913. https://doi.org/10.1080/07391102.1990.10508531
  15. Shibutani S., Takeshita M., Grollman A. P. Nature, 1991, 349: 431–434. https://doi.org/10.1038/349431a0