Complexation of RNA with mannitol, its spectral characteristics and biological activity

TitleComplexation of RNA with mannitol, its spectral characteristics and biological activity
Publication TypeJournal Article
Year of Publication2016
AuthorsVivcharyk, MM, Ilchenko, OO, Levchenko, SM, Tkachuk, ZYu.
Abbreviated Key TitleDopov. Nac. akad. nauk Ukr.
DOI10.15407/dopovidi2016.10.078
Issue10
SectionBiology
Pagination78-83
Date Published10/2016
LanguageUkrainian
Abstract

The spectral characteristics of the complexation between the RNA and ligands such as mannitol, sorbitol, and lactose, by using the methods of FTIR and FT-Raman spectroscopy and the mathematical model MCR-ALS, are studied. The complexation optimal ratios are determined in the RNA ligand system. In the RNA: mannitol mixture, the ratio is 3 : 1 with complexation about 100 %. In the case of a RNA: lactose mixture, the complexation decreases up to 30 %, and for a RNA: sorbitol mixture, up to 25 %. The results are in a good agreement with the antiviral activity of drugs on the cell culture. The optimal ratio and the better complexation in a RNA: mannitol mixture correspond to the best biological activity of this system. As a result of the replacement of mannitol by lactose in the RNA-ligand system, the biological activity of the preparation changes from antiviral preparation to immunomodulating and anti-inflammatory ones.

KeywordsFT-Raman spectroscopy, FTIR spectroscopy, mannitol, RNA-based drug
References: 
  1. Ministry of Health of Ukraine. Nuclex: instruction for use (approved 01.09. 2010 № 752) (in Ukraine).
  2. Andreychyn M.A., Tkachuk Z.Yu., Yosyk Ya.I. Zhurn. NAMN Ukraine, 2013, 19, No 1: 100-103 (in Ukrainian).
  3. Tkachuk Z.Yu., Rybalko S.L., Diadiun S.T., Starosyla D.B. Dopov. Nac. akad. nauk Ukr., 2011, No 4: 182-188 (in Ukrainian).
  4. Tkachuk Z., Dykyi B., Kondryn A., Prishliak O., Vaskul N. Ukr. Med. Almanakh, 2011, 14, No 4: 200-203 (in Ukrainain).
  5. Porva Y., Tkachuk Z.Y., Rybalko S.L. Visnyk farmakolohii ta farmatsii, 2010, No 9: 10-16 (in Ukrainain) .
  6. Dykyi B.M., Hryzhak I.H., Tkachuk Z.Y., Ostiak R.S., Vaskul N.V. Infektsiini khvoroby, 2011, No 4: 31-34 (in Ukrainian).
  7. Tkachuk Z.Yu., Tkachuk V.V., Tkachuk L. V. J. Biopolym. Cell, 2006, 22: 109-116. https://doi.org/10.7124/bc.000723
  8. Pat. 8420617B2, US, Multiantivirus compound, composition and method of treatment of virus di seases, Tkachuk Z., Publ. 11.03.2011.
  9. Qian J., Chandler L., Bergles E. Characterization and Transformation Monitoring of Polymorphs of D-Mannitol with Raman Spectroscopy, Process monitoring with 1064 nm Raman, Copyright BaySpec, Inc., San Jose, 2013.
  10. Frolov V.M., Tkachuk Z.Y., Kruglova O.V. Ukr. Med. Almanakh, 2012, 15, No 4: 217-227 (in Ukrainian).
  11. Manual for the laboratory diagnosis of viral and rickettsial diseases, Moscow: Medicine, 1965 (in Russian).
  12. Aminoff D. Biochem. J., 1961, 81: 384-392. https://doi.org/10.1042/bj0810384
  13. Loprete M., Hartman K.A. Biochemistry, 1993, 32: 4077-4082. https://doi.org/10.1021/bi00066a032
  14. Jaumot J., Gargalloa R., de Juan A., Tauler R. Chemometr. Intell. Lab., 2005, 76: 101-110. https://doi.org/10.1016/j.chemolab.2004.12.007