The effect of laser (384 nm) irradiation of a Lewis lung carcinoma on the tumor volume and the formation of metastases

TitleThe effect of laser (384 nm) irradiation of a Lewis lung carcinoma on the tumor volume and the formation of metastases
Publication TypeJournal Article
Year of Publication2017
AuthorsKanevskyi, VA, Osinskyi, SP, Pushkarev, VM
Abbreviated Key TitleDopov. Nac. akad. nauk Ukr.
Date Published11/2017

We study the effect of laser irradiation upon implanted Lewis carcinoma. The irradiation of mice with Lewis carcinoma by a femtosecond pulsed laser with a wavelength of 384 nm was performed. Four groups of animals with different irradiation schemes were used in the experiments. The tumor volume and the number of lung metastases were detetermined. The activity of matrix metalloproteinases MMP-2 and MMP-9 in the primary tumor tissue was studied using zymography in polyacrylamide gel. The significant inhibition of the primary tumor growth and a decrease in the number of lung metastases were showed. A significant decrease of the MMP-9 level in the tumor was also observed. The most promising is the irradiation of mice with Lewis carcinoma on the 18th day after cells inoculation. There was a significant (more than 2-fold) reduction in the tumor volume, number of metastases in lungs, and MMP-9 level. Laser irradiation with a wavelength of 384 nm may be promising for the treatment of superficial tumors.

Keywordsfemtosecond laser with tunable wavelength, Lewis carcinoma, lung metastases, matrix metalloproteinases 2/9
  1. Carvalho, J. L., Britto, A., de Oliveira, A. P., Castro-Faria-Neto, H., Albertini, R., Anatriello, E. & Aimbire, F. (2017). Beneficial effect of low-level laser therapy in acute lung injury after i-I/R is dependent on the secretion of IL-10 and independent of the TLR/MyD88 signaling. Lasers Med. Sci., 32, pp. 305-315.
  2. Haslerud, S., Lopes-Martins, R. A., Frigo, L., Bjordal, J. M., Marcos, R. L., Naterstad, I. F., Magnussen, L. H. & Joensen, J. (2017). Low-level laser therapy and cryotherapy as mono- and adjunctive therapies for achilles tendinopathy in rats. Photomed. Laser Surg., 35, pp. 32-42.
  3. Liu, Y. & Zhang, H. (2016). Low-level laser irradiation precondition for cardiac regenerative therapy. Photomed. Laser Surg., 34, pp. 572-579.
  4. Eom, Y., Kwon, J., Heo, J. H., Yun, C., Kang, S. Y., Kim, H. M. & Song, J. S. (2016). The effects of proinflammatory cytokines on the apoptosis of corneal endothelial cells following argon laser iridotomy. Exp. Eye Res., 145, pp. 140-147.
  5. Kanevsky, V. A. & Pushkarev, V. M. (2016). Effect of laser irradiation of the thymus projection on the level of cytokines in mice tissues. Dopov. Nac. akad. nauk Ukr., 12, pp. 96-101. doi:
  6. Ren, X., Ge, M., Qin, X., Xu, P., Zhu, P., Dang, Y., Gu, J. & Ye, X. (2016). S100a8/NF-κB signal pathway is involved in the 800-nm diodelaser-induced skin collagen remodeling. Lasers Med. Sci., 31, pp. 673-678.
  7. Snoek-van Beurden, P. A. M. & Von den Hoff, J. W. (2005). Zymographic techniques for the analysis of matrix metalloproteinases and their inhibitors. Biotechniques, 38, pp. 73-83.
  8. Akram, Z., Abduljabbar, T., Sauro, S. & Daood, U. (2016). Effect of photodynamic therapy and laser alone as adjunct to scaling and root planing on gingival crevicular fluid inflammatory proteins in periodontal disease: A systematic review. Photodiagnosis Photodyn. Ther., 16, pp. 142-153.
  9. Min, S., Park, S. Y., Moon, J., Kwon, H. H., Yoon, J. Y. & Suh, D. H. (2016). Comparison between Er:YAG laser and bipolar radiofrequency combined with infrared diode laser for the treatment of acne scars: Differential expression of fibrogenetic biomolecules may be associated with differences in efficacy between ablative and non-ablative laser treatment. Lasers Surg. Med. doi: