Induction of NO synthesis in roots of wheat plantlets and development of their heat resistance by exogenous L-arginine and nitrate

TitleInduction of NO synthesis in roots of wheat plantlets and development of their heat resistance by exogenous L-arginine and nitrate
Publication TypeJournal Article
Year of Publication2017
AuthorsKarpets, Yu.V, Kolupaev, Yu.E, Dmitriev, AP
Abbreviated Key TitleDopov. Nac. akad. nauk Ukr.
DOI10.15407/dopovidi2017.07.077
Issue7
SectionBiology
Pagination77-84
Date Published7/2017
LanguageRussian
Abstract

The treatment of roots of intact plantlets of wheat (Triticum aestivum L.) with L-arginine and sodium nitrate caused an increase of the content of nitric oxide (NO) in them and raised the resistance to the damaging heating. The arginine-dependent increase of the NO content was suppressed by the pretreatment of roots with NO-synthase inhibitor L-NAME (NG-nitro-L-arginine methyl ester), and the nitrate-dependent one — with nitrate reductase inhibitor, sodium tungstate. These inhibitors eliminated also the positive influence of exogenous L-arginine and nitrate on the heat resistance of plantlets that confirms the crosstalk of such influence with the process of nitric oxide synthesis. At the combined treatment of plantlets with L-arginine and nitrate, their influence on the content of nitric oxide in roots and the development of the heat resistance of plantlets was leveled. The oppression of the nitrate-dependent formation of nitric oxide in plantlets roots, caused by L-arginine, was partially removed by NO-synthase inhibitor L-NAME. Thus, the data on the antagonism of the arginine- and nitrate-dependent pathways of nitric oxide synthesis in plant cells are obtained for the first time.

Keywordsheat resistance, L-arginine, nitrate, nitrate reductase, nitric oxide, NO-synthase, Triticum aestivum
References: 
  1. Dmitriev, A. P. (2004). Sygnal role of nitric oxide in plants. Tsitol. Genet., 38, No. 4, pp. 67-75 (in Russian).
  2. Wilson, I. D., Neill, S. J. & Hancock, J. T. (2008). Nitric oxide synthesis and signalling in plants. Plant Cell Environ., 31, No. 5, pp. 622-631. https://doi.org/10.1111/j.1365-3040.2007.01761.x
  3. Khan, M. N., Mobin, M. & Abbas, Z. K. (2015). Nitric oxide and high temperature stress: a physiological perspective. In Khan, M. N. et al. (Eds.). Nitric oxide action in abiotic stress responses in plants (rr. 77-94). Heidelberg; New York; Dordrecht; London: Springer. doi: https://doi.org/10.1007/978-3-319-17804-2_5
  4. Karpets, Yu. V., Kolupaev, Yu. E. & Vauner, A. A. (2015). Functional interaction between nitric oxide and hydrogen peroxide during formation of wheat seedling induced heat resistance. Russ. J. Plant Physiol., 62, No. 1, pp. 65-70. https://doi.org/10.1134/S1021443714060090
  5. Siddiqui, M. H., Al-Whaibi, M. H. & Basalah, M. O. (2011). Role of nitric oxide in tolerance of plants to abiotic stress. Protoplasma, 248, No. 3, pp. 447-455. https://doi.org/10.1007/s00709-010-0206-9
  6. Barand, A., Nasibi, F. & ManouchehriKalantari, Kh. (2015). The effect of arginine pretreatment in the increase of coldtolerance in Pistacia vera L. in vitro. Russ. Agricult. Sci., 41, No. 5, pp. 340-346. https://doi.org/10.3103/S1068367415050043
  7. Mur, L. A. J., Mandon, J., Persijn, S., Cristescu, S. M., Moshkov, I. E., Novikova, G. V., Hall, M. A., Harren, F. J. M., Hebelstrup, K. H. & Gupta, K. J. (2013). Nitric oxide in plants: an assessment of the current state of knowledge. AoB Plants, 5, pls052. https://doi.org/10.1093/aobpla/pls052
  8. Glyan'ko, A. K. & Mitanova, N. B. (2011). Synthesis nitric oxide (NO) in roots etiolated seedlings of pea. Visn. Kharkiv Nat. Agr. Un-ty. Ser. Biol., Iss. 3, pp. 6-14 (in Russian).
  9. Roszer, T. (2014). Biosynthesis of nitric oxide in plants. In Khan, M. N. et al. (Eds.). Nitric Oxide in Plants: Metabolism and Role in Stress Physiology (pp. 17-32). Cham: Springer. https://doi.org/10.1007/978-3-319-06710-0_2
  10. Corpas, F. J. & Barroso, J. B. (2016). Nitric oxide synthase-like activity in higher plants. Nitric Oxide. doi: https://doi.org/10.1016/j.niox.2016.10.009
  11. Crawford, N. M. (2005). Mechanisms for nitric oxide synthesis in plants. J. Exp. Bot., 57, No. 3, pp. 471-478. doi: https://doi.org/10.1093/jxb/erj050
  12. Shi, F.-M. & Li, Y.-Z. (2008). Verticillium dahliae toxins-induced nitric oxide production in Arabidopsis is major dependent on nitrate reductase. BMB Rep., 41, No. 1, pp. 79-85. https://doi.org/10.5483/BMBRep.2008.41.1.079
  13. Karpets, Yu. V., Kolupaev, Yu. E., Yastreb, T. O. & Oboznyi, A. I. (2016). Induction of heat resistance in wheat seedlings by exogenous calcium, hydrogen peroxide, and nitric oxide donor: functional interaction of signal mediators. Russ. J. Plant Physiol., 63, No. 4, pp. 490-498. https://doi.org/10.1134/S1021443716040075
  14. Rosales, E. P., Iannone, M. F., Groppa, M. D. & Benavides, M. P. (2011). Nitric oxide inhibits nitrate reductase activity in wheat leaves. Plant Physiol. Biochem., 49, No. 2, pp. 124-130. https://doi.org/10.1016/j.plaphy.2010.10.009
  15. Vital, S. A., Fowler, R. W., Virgen, A., Gossett, D. R., Banks, S. W. & Rodriguez, J. (2008). Opposing roles for superoxide and nitric oxide in the NaCl stress-induced upregulation of antioxidant enzyme activity in cotton callus tissue. Environ. Exp. Bot., 62, No. 1, pp. 60-68. https://doi.org/10.1016/j.envexpbot.2007.07.006