Role of reactive oxygen and nitrogen species in the induction of heat resistance of wheat plantlets by exogenous hydrogen sulfide

TitleRole of reactive oxygen and nitrogen species in the induction of heat resistance of wheat plantlets by exogenous hydrogen sulfide
Publication TypeJournal Article
Year of Publication2019
AuthorsKarpets, Yu.V, Kolupaev, Yu.E, Yastreb, TO, Horielova, OI, Shkliarevskyi, MA, Dmitriev, OP
Abbreviated Key TitleDopov. Nac. akad. nauk Ukr.
Date Published03/2019

The influence of sodium hydrosulfide (NaHS) as a donor of hydrogen sulfide (H2S) on the heat resistance of wheat plantlets and the possible participation of H2O2 and NO as mediators in the realization of stress protective effects of H2S have been studied. The treatment of plantlets with NaHS increases their survival after the damaging heating. During the first four hours after the beginning of the influence of the H2S donor, an increase in the content of H2O2 and NO in roots is registered. The treatments of plantlets with antioxidant dimethyl thiourea (DMTU), NADPH oxidase inhibitor imidazole, and antagonists of nitric oxide remove the stress protective effect of the H2S donor. At the same time, antagonists of NO only partially inhibit an increase in the content of H2O2 in roots at the NaHS treatment, and DMTU and imidazole almost completely level an increase in the NO content caused by the H2S donor. The results indicate the importance of the preliminary accumulation of H2O2 for an increase of the NO content at the influence of hydrogen sulfide and the participation of both mediators in the realization of its stress protective effects.

Keywordsheat resistance, hydrogen peroxide, hydrogen sulfide, nitric oxide, signal mediators, Triticum aestivum

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