Screening of growth-simulating activity of synthetic compounds - pyrimidine derivatives

TitleScreening of growth-simulating activity of synthetic compounds - pyrimidine derivatives
Publication TypeJournal Article
Year of Publication2020
AuthorsMohilnikova, IV, Tsygankova, VA, Solomyannyi, RM, Brovarets, VS, Bilko, NМ, Yemets, АІ
Abbreviated Key TitleDopov. Nac. akad. nauk Ukr.
Date Published10/2020

New synthetic compounds — pyrimidine derivatives — were screened to select the most effective of them, which show a growth-regulating activity in plants. Their action was compared with phytohormones of the auxin class. In particular, the effect of synthetic compounds on the seed germination, growth, and development of tomato (Solanum lycopersicum L.) plants of Lagidny variety in vivo and in vitro was studied. It was found that some of the studied compounds have a positive effect on the morphometric and biochemical parameters of 30-day-old plants: compounds D1-D5 increased seed germination up to 9-27 %, the most effective among them was compound D1. Compounds D1 and D2 increased the height of the shoots up to 29 %. All the studied compounds effectively influenced the rooting of plants, increasing either the length of the roots (up to 8 %) or their number (up to 25 %), the most effective among them was the compound D2. The effect of these compounds on the morphogenetic potential of tomato explants was studied in vitro for the first time. It was found that compounds D1 and D2 were the most effective for the direct plant regeneration. Screened compounds can be further recommended for the use in various biotechnological programs for genetic improvement of tomatoes.

Keywordsauxins, growth regulators, in vitro, in vivo, plants, pyrimidine derivatives, Solanum lycopersicum L., synthetic compounds

1. Su, Y., Xia, S., Wang, R. & Xiao, L. (2017). Phytohormonal quantification based on biological principles. In Hormone metabolism and signaling in plants (pp. 431-470). London: Acad. Press.
2. Akhtar, S. S., Mekureyaw, M. F., Pandey, C. & Roitsch, T. (2020). Role of cytokinins for interactions of plants with microbial pathogens and pest insects. Front. Plant Sci., 10, 1777.
3. Pramanik, K. & Mohapatra, P. P. (2017). Role of auxin on growth, yield and quality of tomato — a review. Int. J. Curr. Microbiol. Appl. Sci., 6, No. 11. pp. 1624-1636.
4. Vriet, C., Russinova, E. & Reuzeau, C. (2013). From squalene to brassinolide: the steroid metabolic and signaling pathways across the plant kingdom. Mol. Plant., 6, pp. 1738-1757.
5. Muhammad, I. & Muhammad, A. (2013). Gibberellic acid mediated induction of salt tolerance in wheat plants: growth, ionic partitioning, photosynthesis, yield and hormonal homeostasis. Environ. Exp. Bot., 86, pp. 76-85.
6. Rademacher, W. (2015). Plant growth regulators: backgrounds and uses in plant production. J. Plant Growth Regul., 34, pp. 845-872.
7. Solomyannyi, R. N., Shablykina, O. V., Moskvina, V. S., Khilya, V. P., Rusanov, E. B. & Brovarets, V. S. (2019). 8-(Methyl(phenyl)sulfonyl)-2,6-dihydroimidazo[1,2-c]-pyrimidin-5(3Н)-ones and 9-(methyl(phenyl)sul70 fo nyl)-2,3,4,7-dihydro-6H-pyrimido[1,6-a]pyrimidin-6-ones: synthesis and antiviral activity. Chem. Heterocycl. Compd., 55, No. 4-5, pp. 401-407 (in Russian).
8. Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72, pp. 248-254.
9. Buziashvili, A., Cherednichenko, L., Kropyvko, S. & Yemets, A. (2020). Transgenic tomato lines expressing human lactoferrin show increased resistance to bacterial and fungal pathogens. Biocatal. Agricult. Biotechnol., 25, 101602.
10. Liu, X, Zhang, H., Zhao, Y., Feng, Z., Li, Q., Yang, H.-Q., Luan, S., Li, J. & He, Z.-H. (2013). Auxin controls seed dormancy through stimulation of abscisic acid signaling by inducing ARF-mediated ABI3 activation in Arabidopsis. Proc. Natl. Acad. Sci. USA, 110, No. 38, pp. 15485-15490.
11. Woodward, A. W. & Bartel, B. (2005). Auxin: regulation, action, and interaction. Ann. Botany, 95, pp. 707-735.
12. Patel, J. S., Sitapara, H. H. & Patel, K. A. (2012). Influence of plant growth regulators on growth, yield and quality of tomato and brinjal. Int. J. Forestry Crop Improv., 3, No. 2, pp. 116-118.
13. Wang, H., Jones, B., Li, S. H., Frasse, P., Delalande, C., Regad, F., Chaabouni, S., Latche, A., Pech, J. & Bouzayen, M. (2005). The tomato Aux/IAA transcription factor IAA9 is involved in fruit development and leaf morphogenesis. Plant Cell, 17, pp. 2676-2692.
14. Jamous, F. & Abu-Qaoud, H. (2015). In vitro regeneration of tomato (Lycopersicon esculentum Mill). Plant Cell Biotechnol. Mol. Biol., 16, No. 3-4, pp. 181-190.
15. Gubis, J., Lajchova, Z., Farago, J.& Jurekova, Z. (2003). Effect of genotype and explant type on shoot re generation in tomato (Lycopersicon esculentum Mill.) in vitro. Czech J. Genet. Plant Breed., 39, No. 1, pp. 9-14.