Participation of nitric oxide in induction of heat resistance of wheat seedlings by putrescine

TitleParticipation of nitric oxide in induction of heat resistance of wheat seedlings by putrescine
Publication TypeJournal Article
Year of Publication2020
AuthorsKokorev, AI, Kolupaev, Yu.E, Karpets, Yu.V, Dyachenko, AI
Abbreviated Key TitleDopov. Nac. akad. nauk Ukr.
Date Published12/2020

Polyamines are stress plant metabolites involved in the formation of adaptive responses. Recently, they have been considered as compounds that are involved in signaling processes. However, the possible role of nitric oxide (NO) and its functional interaction with reactive oxygen species (ROS) in the realization of stress-protective effects of polyamines has been very poorly studied. The participation of NO as a signaling mediator in the induction of the heat resistance of wheat seedlings by the exogenous diamine putrescine is studied. Incubation of intact seedling roots on a 1 mM putrescine solution caused an increase in the activity of diamine oxidase and a transient increase in the content of nitric oxide and hydrogen peroxide, respectively, with peaks at 1 and 2 hours from the start of the treatment. The increase in the NO content was eliminated by the action of aminoguanidine (an inhibitor of diamine oxidase and NO synthase), but not sodium tungstate (an inhibitor of nitrate reductase). Treatment of seedlings with a scavenger of hydrogen peroxide with dimethylthiourea (DMTU) reduced the effect of increasing the NO content in roots caused by putrescine. At the same time, scavenger NO PTIO (2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide) almost completely eliminated the accumulation of hydrogen peroxide in the roots, which occurred under the action of putrescine. The increase in the heat resistance of seedlings induced by the treatment with putrescine was completely eliminated under the influence of the NO PTIO scavenger, an inhibitor of diamine oxidase and NO synthase aminoguanidine, and the antioxidant DMTU. It is concluded about the functional interaction of ROS and NO as a signal intermediaries, providing the induction of protective responses and the development of plants of heat-resistance in the processing of putrescine.

Keywordsheat resistance, hydrogen peroxide, nitric oxide (NO), polyamines, putrescine, signaling mediators, Triticum aestivum.

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