Individual features of radiation-induced genomic instability in patients with glioblastoma

TitleIndividual features of radiation-induced genomic instability in patients with glioblastoma
Publication TypeJournal Article
Year of Publication2020
AuthorsZemskova, OV, Glavatsky, OY, Kurinnyi, DA, Demchenko, OM, Rushkovsky, SR
Abbreviated Key TitleDopov. Nac. akad. nauk Ukr.
Date Published4/2020

Using the method of Comet assay under neutral conditions, the features of individual radiation-induced genome instability in patients with glioblastoma was studied. It was found that, in the culture of peripheral blood lymphocytes of two patients with pathomorphologically verified glioblastoma (patients No. 1 and No. 3), the frequency of comets with a high level of DNA damage significantly exceeded the values in the cultures of blood lymphocytes of the comparison group. After irradiation in a dose of 1.0 Gy, the frequency of cells with a high DNA damage rate was increased in lymphocyte cultures of patients No. 2 and No. 3 and decreased in patient No. 1. Frequency analysis of the distribution of individual “comets” depending on their levels of DNA damage revealed the presence of a significant pool of cells in the lymphocyte cultures of patients No. 2 and No. 3 which stopped di vision at the S stage of the cell cycle. After the irradiation, the frequency of such cells in patient No. 3 decreased significantly. It was noted that apoptotic activity in cultures of lymphocytes of neuro-oncological patients was significantly higher than in cultures of conditionally healthy volunteers.

Keywordsapoptosis, Comet assay, glioblastoma, human peripheral blood lymphocytes culture, γ-irradiation

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