Influence of carbon monoxide (CO) donor on heat resistance of wheat plantlets and generation of reactive oxygen species by them

TitleInfluence of carbon monoxide (CO) donor on heat resistance of wheat plantlets and generation of reactive oxygen species by them
Publication TypeJournal Article
Year of Publication2020
AuthorsShkliarevskyi, MA, Kolupaev, Yu.E, Karpets, Yu.V, Shvidenko, MV, Dmitriev, OP
Abbreviated Key TitleDopov. Nac. akad. nauk Ukr.
Date Published8/2020

The influence of hemin (donor of carbon monoxide CO formed due to action of heme oxygenase) on the heat resistance of wheat plantlets and the possible participation of reactive oxygen species (ROS) as mediators in the realization of stress-protective effects of exogenous CO have been studied. The treatment of plantlets with hemin in the concentrations of 0.5-5.0 μM caused a significant increase in their resistance to the damaging heating (10 min at a temperature of 45 °C). Under the action of hemin, the transient increase in the content of hydrogen peroxide in roots was observed with the maximum in 2 hours after the treatment start. In addition, the extracellular peroxidase activity increased temporarily. The treatment of roots with the inhibitor of peroxidase — sodium azide — eliminated both the increase in the activity of extracellular peroxidase and the rise in the content of hydrogen peroxide in roots caused by the action of hemin. At the same time, the treatment with the inhibitor of NADPH oxidase — imidazole — had almost no influence on the effect of an increase in the content of H2O2 under the action of hemin. Under the treatment of plantlets with a scavenger of hydrogen peroxide — dimethylthiourea — both the rise in the H2O2 content and the increase in the heat resistance after the action of hemin are not observed. All studied effects of hemin were eliminated under the influence of CO scavenger hemoglobin. The conclusion is made about the role of ROS, generated by extracellular peroxidase, as the mediators in the process of induction of the heat resistance of plantlets by the CO donor.

Keywordscarbon monoxide, heat resistance, hydrogen peroxide, peroxidase, signal mediators, Triticum aestivum

1. Wang, M. & Liao, W. (2016). Carbon monoxide as a signaling molecule in plants. Front. Plant Sci., 7, art. 572.
2. Shekhawat, G. S. & Verma, K. (2010). Haem oxygenase (HO): an overlooked enzyme of plant metabolism and defence. J. Exp. Bot., 61, No. 9, pp. 2255-2270.
3. Liu, Y., Xu, S., Ling, T., Xu, L. & Shen, W. (2010). Heme oxygenase/carbon monoxide system participates in regulating wheat seed germination under osmotic stress involving the nitric oxide pathway. J. Plant Physiol., 167, No. 16, pp. 1371-1379.
4. Verma, K., Dixit, S., Shekhawat, G. S. & Alam, A. (2015). Antioxidant activity of heme oxygenase 1 in Brassica juncea (L.) Czern. (Indian mustard) under salt stress. Turk. J. Biol., 39, pp. 540-549.
5. Cheng, T., Hu, L., Wang, P., Yang, X., Peng, Y., Lu, Y., Chen, J. & Shi, J. (2018). Carbon monoxide potentiates high temperature-induced nicotine biosynthesis in Tobacco. Int. J. Mol. Sci., 19, art. 188.
6. Li, Z.-G., Gu, S.-P. (2016). Hydrogen sulfide as a signal molecule in hematin-induced heat tolerance of tobacco cell suspension. Biol. Plant., 60, pp. 595-600.
7. Chen, Q., Gong, C., Ju, X., Zhu, Z., Shen, W., Shen, Z. & Cui, J. (2018). Hemin through the heme oxygenase 1/ferrous iron, carbon monoxide system involved in zinc tolerance in Oryza sativa L. J. Plant Growth Regul., 37, pp. 947-957.
8. Ling, T., Zhang, B., Cui, W., Wu, M., Lin, J., Zhou, W., Huang, J. & Shen, W. (2009). Carbon monoxide mitigates salt-induced inhibition of root growth and suppresses programmed cell death in wheat primary roots by inhibiting superoxide anion overproduction. Plant Sci., 177, No. 4, pp. 331-340.
9. Meng, D. K., Chen, J. & Yang, Z. M. (2011). Enhancement of tolerance of Indian mustard (Brassica juncea) to mercury by carbon monoxide. J. Hazardous Materials., 186, No. 2-3, pp. 1823-1829.
10. She, X.-P. & Song, X.-G. (2008). Carbon monoxide-induced stomatal closure involves generation of hydrogen peroxide in Vicia faba guard cells. J. Integr. Plant Biol., 50, pp. 1539-1548.
11. Xuan, W., Huang, L., Li, M., Huang, B., Xu, S., Liu, H., Gao, Y. & Shen, W. (2007). Induction of growth elongation in wheat root segments by heme molecules: a regulatory role of carbon monoxide in plants? Plant Growth Regul., 52, pp. 41-51.
12. Kolupaev, Yu. E., Oboznyi, A. I. & Shvidenko, N. V. (2013). Role of hydrogen peroxide in generation of a signal inducing heat tolerance of wheat seedlings. Russ. J. Plant Physiol., 60, No. 2, pp. 227-234.
13. Sagisaka, S. (1976). The occurrence of peroxide in a perennial plant, Populus gelrica. Plant Physiol., 57, No. 2, pp. 308-309.
14. Minibayeva, E. V., Gordon, L. K., Kolesnikov, O. P. & Chasov, A. V. (2001). Role of extracellular peroxidase in the superoxide production by wheat root cells. Protoplasma, 217, No. 1-3, pp. 125-128.
15. Sa, Z.S., Huang, L. Q., Wu, G. L., Ding, J. P., Chen, X. Y., Yu, T., Ci, S. & Shen, W. B. (2007). Carbon monoxide: a novel antioxidant against oxidative stress in wheat seedling leaves. J. Integr. Plant Biol., 49, pp. 638-645.