Neuropeptide kyotorphin prevents the development of apoptosis of fibroblasts L929 under cold stress
Keywords:cold stress, apoptosis, kyotorphin, fibroblasts L929
We were study the antiapoptotic effect of the neuropeptide kyotorphin in a model of cold stress fibroblasts L929. The effect of dipeptide on cells was analyzed by morphological parameters, using cytofluorimetry with simultaneous staining of annexin V/7-AAD and study of the release of cytochrome c to the cytoplasm of cells. Under selected conditions of cold stress, there was a significantly increase in the number of cells with morphological features of apoptosis according to light microscopy, violation of membrane asymmetry, and the appearance of cytochrome c cells in the cytoplasm was registered. It has been proved that the addition of kyotorphin at a concentration of 100 μg/l to the culture medium 15 min before the modeling of cold stress significantly reduces the number of cells features of apoptosis. The number of annexin V+/7-AAD–-cells after pre-incubation of fibroblasts with dipeptide decreased to 19.1 ± 0.5 % compared to samples after cold stress (55.3 ± 2.1 %), and the number of cells in the cytoplasm of which was registered cytochrome c, decreased by 3.65 times. Studies have suggested that pre-incubation with the regulatory neuropeptide kyotorphin at a concentration of 100 μg/l effectively prevents apoptosis in fibroblasts L929 under cold stress.
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