Phosphorylation of structural viral proteins — essential condition for plant viruses reproduction
Keywords:potato X-virus, structural protein of PVX, phosphorylation, protein kinases
Phosphorylation of proteins is characteristic for structural proteins of cauliflower mosaic virus, potato virus A (PVA), plum beetle virus, and others. This process is necessary for the development of viral infection in the plant. Potato virus X (PVX) is a typical member of the genus Potexvirus. It was found that the structural viral protein of potato virus X is able to phosphorylation in a standard incubation medium in vitro, which contained (gamma-32P)ATP. In contrast, PVX protein in a standard incubation medium in the presence of (alpha-32P)ATP is not phosphorylated. Thus, the final ATP phosphate plays a major role in the phosphorylation of the structural PVX protein. Phosphorylated PVX protein in the system in vivo was detected by exposing nitrocellulose with immunoblot on X-ray film PM-1. As a result, an autograph of the phosphorylated in vivo structural protein PVX was obtained. The concentration of Ca and EDTA ions in the in vitro phosphorylation system affects the process of phosphorylation of the structural protein of PVX . The presence of 10 mM EDTA and 10 mM CaCl2 in the in vitro phosphorylation system inhibited the phosphorylation process of the PVX structural protein. The phenomenon of phosphorylation of the structural protein of PVX is probably used in the processes of virus reproduction in an infected cell.
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