Gadolinium orthovanadate nanoparticles increase survival of old rats

TitleGadolinium orthovanadate nanoparticles increase survival of old rats
Publication TypeJournal Article
Year of Publication2020
AuthorsNikitchenko, YV, Klochkov, VK, Kavok, NS, Karpenko, NA, Sedyh, OO, Bozhkov, AI, Malyukin, YV, Semynozhenko, VP
Abbreviated Key TitleDopov. Nac. akad. nauk Ukr.
SectionMaterials Science
Date Published2/2020

The effect of europium-activated gadolinium orthovanadate nanoparticles (GdVO4 : Eu3+ NPs, 8x25 nm) on the survival, thyroxine concentration, body temperature, and prooxidant-antioxidant balance in liver and blood of aging rats was studied. It was found that the application of GdVO4 : Eu3+ NPs with drinking water (0.25— 0.3 mg/kg of weight per day) from 22-month-old age leads to a significant increase in the survival. The survival medians of control and experimental rats were 900 and 1010 days. A decrease in the rectal temperature and the preservation of the thyroxine concentration in the blood at the level of younger animals were noted: 50.1 ± 1.9 nmol / L vs. 41.4 ± 3.3 nmol / L in the control (p < 0.05). The normalization of the prooxidant-antioxidant balance in liver and blood of aging animals has been established. So, in 33 month old rats treated with nanoparticles, the level of lipid hydroperoxides in serum and liver mitochondrial fraction decreased and did not differ from the indices of 12 month old control rats. Apparently, this effect was facilitated by an increase in the activity of the glutathione peroxidase antioxidant system and glutathione reductase activity in the postmitochondrial fraction of liver to the level of 12 month old rats under the influence of nanoparticles. The data obtained indicate the pro mise of GdVO4Eu3+ NPs for the development of geroprotective drugs that increase the life expectancy of an aging organism.

Keywordsbody temperature, GdVO4 : Eu3+, nanoparticles, prooxidant-antioxidant balance., survival of old rats, thyroxinе

1. Harman, D. (2001). Aging: overview. Ann. N.Y. Acad. Sci. 928(1), pp. 1-21. Doi:
2. Sohal, R. S. (2002). Role of oxidative stress and protein oxidation in the aging process. Free Radical Biology and Medicine. 33(1), pp. 37-44. Doi:
3. Finkel, T., Holbrook, N. J. (2000). Oxidants, oxidative stress and the biology of ageing. Nature, 408(6809), p. 239. Doi:
4. Melov, S. (2002). Therapeutics against mitochondrial oxidative stress in animal models of aging. Ann. N.Y. Acad. Sci. 959(1), pp. 330-340. Doi:
5. Nikitchenko, Yu. V. (2012) Prooxidant-antioxidant system in ageing processes and experimental approaches to its correction (Unpublished doctor thesis). Karasin National University, Kharkov, Ukraine (in Ukrainian).
6. Kim, J., Takahashi, M., Shimizu, T., Shirasawa, T., Kajita, M., Kanayama, A. & Miyamoto, Y. (2008). Effects of a potent antioxidant, platinum nanoparticle, on the lifespan of Caenorhabditis elegans. Mechanisms of ageing and development, 129(6), pp. 322-331. Doi:
7. Quick, K. L., Ali, S. S., Arch, R., Xiong, C., Wozniak, D. & Dugan, L. L. (2008). A carboxyfullerene SOD mimetic improves cognition and extends the lifespan of mice. Neurobiology of aging, 29(1), pp. 117-128. Doi:
8. Narayanan, K. B. & Park, H. H. (2013). Pleiotropic functions of antioxidant nanoparticles for longevity and medicine. Advances in colloid and interface science. 201, pp. 30-42. Doi:
9. Tripathi, D., Mani, V. & Pal, R. P. (2018). Vanadium in biosphere and its role in biological processes. Biological trace element research. 186(1), pp. 52-67. Doi:
10. Karpenko, N. A., Malukin, Yu. V., Koreneva, E. M., Klochkov, V. K., Kavok, N. S., Smolenko, N. P. & Pochernyaeva, S. S. (2013, September). The Effects of Chronic Intake of Cerium Dioxide or Gadolinium Ortovanadate Nanoparticles in Aging Male Rats. Proceedings of the 3rd Int. conf. Nanomaterials: Applications and Properties, 2013, (pp. 04NAMB28-1–04NAMB28-4), Alushta.
11. Averchenko, E. A., Kavok, N. S., Klochkov, V. K., & Malyukin, Yu. V. (2014). Chemiluminescent Diagnostics of Free-Radical Processes in an Abiotic System and in Liver Cells in the Presence of Nanoparticles Based on Rare-Earth Elements nReVO4 : Eu3+(Re  Gd, Y, La) and CeO2. J. Appl. Spectrosc., 81(5), pp. 827-833. Doi:
12. Klochkov, V. K., Malyshenko, A. I., Sedykh, O. O., & Malyukin, Y. V. (2011). Wet chemical synthesis and characterization of luminescent colloidal nanoparticles: ReVO4 : Eu3+(Re - La, Gd, Y) with rod-like and spindle-like shape. Functional materials, pp. 111-115.
13. Klochkov, V. K., Grigorova, A. V., Sedyh, O. O. & Malyukin, Yu. V. (2012). Characteristics of nLnVO4 : Eu3+ (Ln - La, Gd, Y, Sm) sols with nanoparticles of different shapes and sizes. J. Appl. Spectrosc., 79(5), pp. 726-730. Doi:
14. Bozhkov, A. I. & Nikitchenko, Yu. V. (2013). Caloric restriction diet induces specific epigenotypes associated with life span extension. J. Nutrition. Therapeut., 2(1), pp. 30-39. Doi:
15. Belostotskaia, L. I., Dziuba, V. N. & Nikitchenko, I. (2008). The effect of three different hypocaloric diets on oxidative phosphorylation and activity of enzymatic antioxidant system in rat liver mitochondria. Advances in gerontology - Uspekhi gerontologii, 21(2), pp. 235-239 (in Russian).